Coronary Heart Disease With Blood Stasis Syndrome And FgB?-148C/T Gene Polymorphism Correlation Study

Background Coronary heart disease(CHD),a polygenic inheritable disease,is subject to the influences of both genetic and environmental factors.The incidence rate of CHD tends to go up in China with the extension of the average life expectancy,the effective control of acute infectious diseases and the gradual improvement of social economy and life.As the researches on the pathogenesis of CHD go deeper,more and more attention has been paid to the research to such new risk factors as the content of fibrinogen(Fg)and genetic factors.From the perspective of traditional Chinese medical science,heart vessel blockage stasis is the basic pathogenesis of CHD of which blood stasis is the most common syndrome.Many studies have indicated that the Fg content is affected by its gene polymorphism which may be one of the molecular biomarkers of CHD blood stasis syndrome.Objective Discussion on the correlation between single nucleotide polymorphism(SNP)in gene promoter FgB ?-148C/T and CHD blood stasis.Methods Select 100 CHD patients with blood stasis syndrome,50 CHD patients without blood stasis syndrome and 50 healthy people.Extract their peripheral anticoagulated whole blood leucocytes DNA,amplify the target gene segments by polymerase chain reaction(PCR)and detect the gene polymorphism of FgB ?-148C/T genotype by agarose gel electrophoresis(AGE)after the enzyme cutting with Hind III.Carry out statistic analyses based on the results to study their correlations with CHD blood stasis syndrome.Results1.Genotypes of sample FgB ?-148 gene are as follows:64%CC,16% TT and 10%CT for the healthy group,53%CC,26%TT and 21%CT for the CHD patients with blood stasis syndrome and 56%CC,24%TT and 20%CT for the CHD patients without blood stasis syndrome.?The difference between the observed values of distribution and the expected values is of no statistical significance by comparison.(P=0.853)2.It is of statistical significance by comparison for the differences of FgB ?-148C/T genotypes between the CHD patients with blood stasis syndrome and the healthy group(x2=7.19,P<0.05)and between the CHD patients without blood stasis syndrome and the healthy group(x 2=6.804,P<0.05).However,the distribution difference between the CHD patients with blood stasis syndrome and the CHD patients without blood stasis syndrome is of no statistical significance by comparison(x2=0.3294,P>0.05),neither is the distribution difference of alleles C and T on the two groups(P>0.05).The TT genotype may be the independent risk factor for CHD.For people with a TT genotype,the OR value is 2.833 for them to get CHD with blood stasis syndrome(1.405-5.710)(P=0.003)and is 2.263 for them to get CHD without blood stasis syndrome(P=0.045).The distribution difference of alleles C and T on all the three groups is also of no statistical significance(P>0.05).3.As it is of no statistical significance(x2=0.737,P=0.947>0.05)for the difference of blood stasis syndrome integral constituent ratios among the three genotypes of CHD with blood stasis syndrome,FgB?C/T gene polymorphism thus is considered unrelated to the blood stasis syndrome of patients.Conclusions1.The genotype and allele distribution frequency of all the subjects of the study are the same with the general local population.2.CT and TT genotypes of FgB?-148C/T of CHD with blood stasis syndrome and CHD without blood stasis syndrome in this study are higher than that of the normal population.That gene T may be correlated with CHD incidence is presented.?3.C/T polymorphism of CHD with blood stasis syndrome is of no significant correlation with the blood stasis syndrome integration and alleles C and T are also of no significant correlation with CHD with blood stasis syndrome.