| Objective To observe the clinical effect of Tianhong Oral Liquid and explore its mechanism by observing its influence on rat models of focal cerebral ischemia-reperfusion injury made by thread occlusion of middle cerebral artery. 1 Clinical Study 60 patients differentiated as stagnation of wind-phlegm and stasis with ACI were randomly divided into two groups: the treated group with Tianhong Oral liquid and western medicine and the control group treated only with western medicine for two weeks. Before and after treatment, we evaluated the function of neural system and differentiation respectively in two groups and gave scores. After treatment some indexes such as CBF,PAgT and GMP-140 were measured.2 Experimental Study 78 SD rats were divided into 6 groups at random as follows: sham operation group (S group), ischemia-reperfusion group (Model group, M group), Naoxintong group (N group), high dosage of Tianhong group (TH group), middle-dosage of Tianhong group (TM group), lowdosage of Tianhong group (TL group), with thirteen rats in each group. The animal model of focal cerebral ischemia-reperfusion was made by the method of ischemia for four hours, then reperfusion for six hours. The operative steps of sham operation group were the same as other groups except thread occlusion. The latter four groups had been given drugs for ten days before making the models, while the sham operation group and model group were given 0.9% sodium chloride solution. Neurological ratings were respectively made at the end of ischemia and reperfusion. The animals were sacrificed at the end of reperfusion and their brains were acquired on ice swiftly. The brain was cut coronarily into three parts: the section of optic chiasm (about 2mm), the slice before section of optic chiasm (about 2mm), and the slice after section of optic chiasm (about 4mm). The section of optic chiasm was stained by 2%TTC, and its percentage of the section of infarction area was counted by applying images processing software. The cerebral cortical tissue at the infarction margin of the section before optic chiasm was dissociated about 100mg and made into neuronal suspension by zymolysis. Then the intracellular calcium ([Ca2+]i) concentration of neurons was measured by laser confocal scanning microscope (LCSM), represented by the mean intracellular fluorescent intensity. On the ice , the cerebral cortical tissue at the infarction margin of the section after optic chiasm was rapidly dissociated about 100mg, then made into brain homogenate in low temperature. The high performance liquid chromatography (HPLC) was used to determine the Glu, Asp and Gly contents in homogenized brain tissue. ResultsI Clinical Study Compared with the control group, in the treated group the evaluation of TCM (Traditional Chinese Medicine) syndromes showedobvious improvement and CBF was higher, but the PAgT and contents of GMP-140 were lower. The differences between two groups were significant (P<0.01).2 Experimental Study The intracellular fluorescent intensity of cortical neurons in other groups was stronger than that of S group, and the contents of Asp and Gly increased in homogenized cortical tissue, and there were significant differences among them (P<0.05 or P<0.01). In comparison with M group, TL group, N group, percentage of infarction area in TH group was lower, the intracellular fluorescent intensity of neurons decreased, the contents of Asp and Gly in homogenized cortical tissue reduced, there were significant differences among them (P<0.05 or P<0.01). In comparison between TH group and TM group, the differences of all indexes were not significant (P>0.05), and there were no significant differences in comparison between TL group and N group (P>0.05).Conclusions Tianhong oral liquid could improve neuronal cell function, prevent and treat ischemia reperfussion injury, treat cerebral infarction , and is worth developing and applying. Its mechanism may lies in: 1. increasing CBF, improving platelet function, inhibiting p...
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