| The adhesion of tendon is related to the transplantation material, especially related to whether the synovial membrane presence on transplanted tendon surface and whether the synovial bursa is intact. There are fewer adhesions on synovial tendons than non-synovial tendons after transplantation. Since the position of synovial tendons are within tendon sheath, taking use of synovial tendons is difficult and destructive procedure, and it may limit the widespread using of synovial tendons in clinical field. Enlightened by the fact that synovial tissue structure will appear around silica gel when buried in subcutaneous tissue, we bury a silica gel bag in rabbit subcutaneous tissue on the animal back at first, and massage it regularly. When the synovial line structure appeared around the silica gel bag, we observed the bursa figure, studied its histology and ultra structure, detected the activity of uridine diphosphoglucose dehydrogenase (UDPGD) in synovial lining structure. At the same time, the expression of vascular cell adhesion molecule 1 (VCAM-1), CD68 and UDPGD mRNA in cells was detected, the character of liquid in subcutaneous synovial bursa was studied, and hyaluronic acid (HA) concentration of liquid was detected with ELISA. Secondly, a portion of peroneus longus tendon (PLT) was taken from self-body of rabbit and it be placed into the artificial subcutaneous synovial bursa. After being cultured in vivo, the changes of tendon surface were observed. Thirdly, the synovialized tendons were replaced to flexor tendon within synovial sheath, and the process of tendon healing was observed. To evaluate the effect of anti-adhesion after tendon transplantation, mean score of adhesion was calculated; the flexion angle of proximal phalange joint (PIP) and the excursion of graft tendon on loads were measured. In addition, the human embryo tendon cells and synoviocytes were cultured in Dulbecco's modified Eagles medium (DMEM), in which 10%FBS, penicillin-streptomycin and amphotericin-B were prepared confluent, both of the cells shape and growth process of the cells were observed with phase-contrast microscopy, the growth curve of cells was drawn, the fission index of cells was calculated, typeâ… and â…¢ collagen, VCAM-1, CD68 were studied by using immunohistochemistry (IHC) staining, the activity of UDPGD was detected.After culture medium was replaced by different concentration of synovial fluid, the tendon cells' growth was observed, the BrdU was incorporated into DNA, and the proliferation of cell was studied by calculating the index of BrdU labeling. After this, the cell type was studied by detecting typeâ… collagen, VCAM-1 and CD68 using immunohistochemistry staining, and the activity of UDPGD in cultured cells was detected at the same time. The results are as follows:1. The artificial subcutaneous synovial bursa model is made.After 2 weeks, a synovial bursa structure appears around the silica gel bag, its internal surface is smooth in whole figure, and the wall of synovial bursa is thick and grey, in histology the artificial subcutaneous synovial bursa consists of synovial like cells and collagen. Synovial microvillus structure was found under scanning electron microscope, and the bursa lining cells have the same ultra structure as synoviocytes. The lining cells express high activity of UDPGD. VCAM-1 and CD68 positive cells were found in bursa wall, UDPGDmRNA expressed in these cells plasm, there was some fluid which like synovial fluid in bursa, the fluid is clearing, no cells were found, the concentration of HA is higher than blood serum.2. Non-synovial tendon is changed to synovial tendon in vivo.At the first week, a smooth membrane structure appears on the surface of non-synovial tendon, and the membrane cells express high activity of UDPGD, VCAM-1 and CD68 positive cells were found in the membrane of synovial-changed tendon. Under scanning electron microscope, micropore structure was found on synovial-changed tendon, the membrane cells of synovial-changed tendon have the same ultra structure li...
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