Study On Pharmacodynamics And Quality Control Methods Of Xinshu Oral Liquid By Using Capillary Electrophoresis

Xinshu oral liquid is extracted and prepared from Radix Angelicae sinensis, Rhizoma Chuanxiong and Flos Carthami. It is commonly used for the treatment of coronary heart disease, angina pectoris, arteriosclerosis and high cholesterol. In this thesis, quality control method of Radix Angelicae sinensis, Rhizoma Chuanxiong, Flos Carthami and Xinshu oral liquid by capillary electrophoresis was developed. The pharmacodynamics of Xinshu oral liquid were studied to provide scientific basis for clarifying the mechanism of therapeutical effect.Firstly, a study on quality control method of Radix Angelicae sinensis was carried out. Extraction methods were evaluated for the extraction efficiency of adenosine and ferulic acid, as well as for the number of peaks in the electropherogram. In this experiment, three factors were studied by orthogonal design, including ethanol concentration, consumption of solvent, and time of extraction. The optimal extraction condition was 60 % ethanol consumed ten times amount of raw material, the extraction repeated three times and each time for 20 minutes. Then, a method for determining adenosine and ferulic acid in Radix Angelicae sinensis was developed.Based on electropherogram of 10 batches of genuine Radix Angelicae sinensis from Minxian, Gansu, China, a standard electrophoretic fingerprint was established. Peaks existing in all 10 electropherograms were assigned as "common peaks" for Radix Angelicae sinensis. There are 24 "common peaks" in the fingerprint. Thestandard profile was used to assess the quality consistency of Radix Angelicae sinensis from 11 different sources. Good similarities with correlation coefficients above 0.9 were found in fingerprints between the raw material from Minxian and those from other sources except those from Ningxia and Qinghai. By comparing the fingerprints, Radix Angelicae sinensis was well distinguished from Radix Levistii, a substitute of Radix Angelicae sinensis in traditional Chinese medicine, and from 8 species of Umbelliferae plants including Rhizoma Chuanxiong, Radix Bupleuri, Radix Saposhnikoviae, Rhizoma Notopterygii, Radix Angelicae pubescentis, Rhizoma Ligustici, Radix Angelicae dahuricae and Radix Peucedani. In addition, a CE-MS fingerprint of Radix Angelicae sinensis was investigated.Secondly, a study on quality control method of Rhizoma Chuanxiong was performed. The extraction methods were evaluated for the extraction efficiency of adenosine and ferulic acid, as well as for the number of peaks in the electropherogram. In this experiment, three factors were studied by orthogonal design, including ethanol concentration, consumption of solvent, and time of extraction. The optimal extraction condition was 60 % ethanol consumed ten times amount of raw material, the extraction repeated three times and each time for 20 minutes. Then, a method for determining adenosine and ferulic acid in Rhizoma Chuanxiong was fulfilled.An average electrophoretic profile was served as the standard fingerprint based on 10 batches of genuine Rhizoma Chuanxiong from Guanxian, Sichuan, China. There are 17 "common peaks" in the fingerprint. The standard profile was used to assess the quality consistency of Rhizoma Chuanxiong from 1 1 different sources. Good similarities were found in fingerprints between the raw material from Guanxian and those from other sources except those from Zhejiang and Yunnan. By comparing the fingerprints, Rhizoma Chuanxiong was well distinguished from 8 species of Umbelliferae plants including Radix Angelicae sinensis, Radix Bupleuri, Radix Saposhnikoviae, Rhizoma Notopterygii, Radix Angelicae pubescentis, Rhizoma Ligustici, Radix Angelicae dahuricae and Radix Peucedani. Moreover, a CE-MS fingerprint of Rhizoma Chuanxiong was investigated.Thirdly, a method for quality control of Flos Carthami was developed. Theextraction methods were evaluated for the extraction efficiency of adenosine, rutin and quercetin, as well as for the number of peaks in the electropherogram. Three factors were studied by orthogonal design,...