The Change Of Expressing Integrin β1, αvβ3 In Detached And Reattached Rabbit Retina

Integrin family is the most important cellular adhesion molecule, which mediates the adhesion of cell-cell or cell-extracellular matrix through the linkage with neighboring cell or extracellular matrix such as fibronectin, laminin or fibrinogen. As an important membrane receptor, integrin can communicate extracellular microenvironment with cellular metabolism to regulate cell growth, tissue repairment, tumor development and metastasis. The study is to set up rabbit retinal rhegmatogenous detachment and reattchment model and investigate the expession change of integrin β1 and integrin α v β 3 in detached and reattched retina. The change of integrin β 1 and α v β 3 of human RPE during wound healing is also studied.The purpose of the first part in this study is to set up a rabbit rhegmatogeneous retinal detachment and reattachment model with vitreous injection of hyalidase and subretinal injection of liquefied vitreous using microtube. Under optical microscopy, an evident loss of photoreceptors, proliferation of retinal pigment epithelial cell and Mliller cell was observed. In the second and the third part, the expression of integrand β 1 and α v β 3 were investigated with immunohistochemistary method and hybridization in situ. The integrin β 1 and α v β 3 was expressed weakly in ganglion cell layer and internal nuclear layer of normal retina. After retinal detachment, the expression of integrin β 1 and α v β 3 was upregulated in neural retina and retinal pigment epithelial cell after retinal detachment but decreasedafter retinal reattchment. In the fourth part, the wound-healing model of human RPE was set up. The expression of integrin β 1 and α v β 3 on cultured human retinal pigment epithelial cell after injury were stronger than that after wound healing.In conclusion, Rabbit retinal detachment and reattchment model were set up successfully with subretinal injection of liquefied vitreous. Our study proved that integrin β 1 and α v β 3 could be expressed weakly in normal rabbit retina to keep the structure entity. The expression of these two integrins was stronger and longer in every layer of detached retina than in reattached retina. The change of integrin β 1 and α v β 3 was simultaneous with pathological change after retinal detachment. Therefore integrin β 1 and α v β 3 participated the wound-healing procession of retina and facilitated the proliferation and migration of the cells. In wound-healing model of cultured human RPE cell, the change of integrins implied that the integrins were involved in mechanisms of contact inhibition of human RPE cells.