| Liver carcinoma is one of most common malignant tumor in China and its death rate is in the second of all malignant tumors. It has such characteristics as not easy to find out and diagnose in its early period, difficult to prevent and cure and low survival rate etc. Liver carcinoma has become one of the most important subject in medical research of China and one of most hotspots in the field of molecule biology.The essential of the development of malignant entity tumor is to lose control of cell growth, the basic reason about it is the abnormity of genes related cancer and special genes, which make the structure and function of these gene changed and lead to metabolizability changed. For the occurrence and development of liver cancer has its particular reason and rule, only the research on molecule level can resolve the problem of its early diagnosis, which will benefit to the clinic for more time and space and higher living rate. This paper is focused on study of the marked test indexes of liver cancer such as a inhibitory gene P16, IGF-II gene related cancer and AFP in a way or a combined way in order to make out the mechanism between marked substances and early diagnosis, to set up a combined test system based on marked substances, to improve the rate of positive test and to provide theoretical basis and clinic standard for early diagnosis of liver carcinoma.The paper proposes bioluminescence immunoassay technology (BLIAT) to test AFP in the serum as a proverbial marked substance for the early diagnosis of liver carcinoma. A biological luminous system consisted of the bacterial luciferase distilled with oceanic photobacterium phosphoreum 502, AFP antibody marked by G6PDH and its anti - antibody is fixed in a solid state through Sepharose – 4B, which will improve the catalytic activity of bacterial luciferase and immunoreaction activity of antigen and antibody. The concentration of AFP in serum of liver carcinoma is examined through BLIAT and the result shows that the positive examination rate is over 90%, which shows that BLIAT has good sensitivity to test AFP. BLIAT has such characteristics as safe, easy, minim and quick ect., it is the only technology with good quality compared favourably with the irradiation immunity method, which has irradiation pollution. It is especially suitable for the carcinoma patient with30%~40% AFP keeping increasing in low concentration and early diagnosis for small liver carcinoma. AFP to be a idiosyncratic marked substance in early diagnosis of liver carcinoma is only the production in the particular period and reflects the regularity in one phase, it has relationship with the occurrence and development of liver carcinoma, has practicability for general survey of liver carcinoma and provide a new means to early diagnosis.The paper discusses the possibility of DNA polymorphism of P3 promoter of IGF-II(insulin like growth factor II) gene to be a idiosyncratic marked substance for early diagnosis of liver carcinoma and relationship between DNA polymorphism change of P3 promoter DNA of IGF-II gene and the occurrence and development of liver carcinoma with the PCR-RFLP method. The enzyme slices of BstEII, EcoRI, Sac II, BglII and AvaII show that there are no the variation of alkaline radical in the points of enzyme slices of EcoRI, Sac II, BglII and AvaII, this shows that they have no relationship with carcinoma, whereas a positive result appeared on the point of BstEII enzyme slice, its positive test rate is 63.3%, which is totally different from the benign tumor, that accounts for DNA polymorphism change of P3 promoter of IGF-II gene has great relationship with the occurrence and development of liver cancer and abnormity DNA of P3 promoter of IGF-II gene maybe first appear than other index. P3 promoter DNA of IGF-II gene is different from AFP in the embryo protein of liver carcinoma and can be expressed before the occurrence of liver carcinoma, so its idiosyncrasy is strong. Therefore DNA polymorphism change of P3 promoter of IGF-II gene to be a idiosyncrat...
|
PDF Full Text Request