The Expressions Of Wnt2in Hepatocellular Carcinoma And The Effect Of Wnt2Silence On Hepatocellular Cell Line HepG2

Objective To explore the expressions of Wnt2in humanhepatocellular carcinoma(HCC) and the suppressive effect of knockingdown Wnt2expression by RNAi on Hepatocellular cell line HepG2.Methods Real-time quantitative PCR (RT-qPCR) method andimmunohistochemistry method were used to detect the expression of Wnt2in HCC, paired Paracancerous tissue and normal liver tissue;RNAi wasused to knock down the expression of Wnt2in HepG2,and the change ofWnt2was detected by RT-qPCR and Western blot,and testing RNAi cellproliferation level and cycle change.Result Wnt2expression is the highest in cancer, lower inpairedParacancerous tissue,and lowest innormal liver. Using RNAitechnology,the expression of Wnt2was downregulated.HepG2proliferationwas inhibited, the cell growth was mainly detained in G0/G1, and thepercentage of S decreased.Conclusion The expression of Wnt2was higher in tumor tissues thanin normal liver tissues; RNAi technology efficiently knocks down the expression of Wnt2in Hepatocellular cell line HepG2,resulting in thesuppression of tumor cell growth.siRNA against Wnt2is a potentialtherapeutic target for Human Hepatocellular Carcinoma.