Research Of The The Characterastics Of Chinese Medical Syndromes Of Chronic Atrophic Gastritis And Machanisms Of Xiaopi Granular's Treatment Effects On CAG And Dysplasia

Gastric carcinoma is the most common tumor in China, early detection, diagnosis, and treatment will greatly decrease mortality rate. There is a long period time of precancerous condition before the normal mucosa change into carcinoma. Detecting and treating precancerous lesions is a good way to prevent gastric carcinoma. Chronic atrophic gastritis and dysplasia is precancerous lesion indicated by WHO, Traditional chinese medicine has good curative effects on it. My thesis can be divided into three parts, I summarized the cognition of cause and mechanisms of chronic atrophic gastritis in the view of traditional chinese medicine, and the development in the research of the etiological factor , pathogenesis, therapeutics of western medicine. In part 2, Using the method of epidemiology and statistics, collecting and studying clinical cases to explore the characteristics of traditional chinese medicine syndrome of chronic atrophic gastritis. In part 3, on the basis of replicating animal model of chronic atrophic gastritis, using technique of immunohistology to study proliferation, apoptosis, angiogenesis, effects of Xiaopi Granule on the expression of the gene correlated with proliferation and apoptosis, angiogenesis and its regulating factors. Result Clinical research Distribution of clinic data: The mean age of patients was approximate to 56a, There was no significant difference in the proportion of gender among groups of different age(P<0.05), the incidence of CAG increased with age(P<0.01), Whether IM or/and Dys existed, there was no significant difference existed in the incidence of IM/Dys between different gender(P>0.05), but incidence of IM/Dys increased with growth of age and course of disease. No significant difference existed between different gender in the infected rate of Hp, infected rate of Hp had no significant correlation with age, infected rate of Hp decreased with the growth of the course of disease. Characteristics of TCM syndrome : Using the factor analysis method, 5 TCM syndromes of CAG were found; There was no significant statistical difference in gender and age among clinical categorizations of CAG(P>0.05). Course of disease was correlated with categorization(P>0.05); the syndrome of deficiency in Spleen-Qi and stagnation in blood,the syndrome of deficient cold accompanied by blood stagnation had longer course of disease. Deficiency in spleen and stomach and stagnation in blood was the most common pathogenesis of CAG, infection of Hp and incidence of IM/Dys had no significant relationship with TCM categorization(P>0.05). Experiment study Experiment 1 The positive expression of PCNA in gastric mucosa of the rats in model group and spontaneous recovery group was significantly higher than in normal group, The positive expression of PCNA was significantly decreased after treated with Xiaopi granule, there was siginificant difference between spontaneous recovery group and Xiaopi granule group. No significant difference showed between control group treated with Weimeisu. Inhibiting the positive expression of PCNA may be one of the mechanisms of Xiaopi granule's treatment effects on CAG and dysplasia. Experiment 2 The positive expression of Bcl-2 in gastric mucosa of the rats in model group and spontaneous recovery group was significantly higher than in normal group, whereas the positive expression of Bax was decreased. After treated with Xiaopi granule, The positive expression of Bcl-2 was strengthened and the positive expression of Bax was weakened. Inhibiting the positive expression of Bcl-2 and strengthening the positive expression of Bax may be one of the mechanisms of Xiaopi granule's treatment effects on CAG and dysplasia. Experiment 3 The MVC, the positive expression of VEGF, the positive expression of FGF in gastric mucosa of the rats in model group and spontaneous recovery group was significantly higher than in normal group, After treated with Xiaopi granule, MVC was decreased, the positive expression of VEGF and FGF was weakend, there was siginificant difference in MVC the positiv...