Study On The Relationship Between The Expression Of P53, PCNA, CerbB-2, The Nuclear DNA Content And Syndromes Of Traditional Chinese Medicine Of Chronic Atrophic Gasritis With Dysplasia

Purpose: Through observing the variety of the expression of p53, PCNA, CerbB-2 and thenuclear DNA content in the syndromes of Traditional Chinese Medicine(TCM) of chronicatrophic gastritis with dysplasia, to study the difference of the syndromes of TCM in thepathologic histology and molecule biology level. Exploring objective and quantitative indexesof syndromes of TCM may be as a guidance to TCM in preventing and treating the chronicatrophic gastritis with dysplasia.Subjects and Methods: 146 cases with chronic atrophic gastritis with dysplasia were dividedinto five types according to the Syndrome Differentiation of TCM. The nuclear DNA contentwas quantatively determined by Feulgen dying method and image analysis technique.Meanwhile S-P immunohistochemical method was applied to detected the expression of p53,PCNA, CerbB-2 in stomach mucosa.Results:1. There ever was the positive expression of oncogenes in the chronic atrophic gastritis withmild dysplasia, but no significant difference was found in the expression of p53 and PCNAamong mild, moderate or severe dysplasia(P>0.05).While the expression of CerbB-2 betweenmild and severe dysplasia had very significant difference(P<0.01).2.The nuclear DNA content increased with the increment of the severity of dysplasia in gastricmucosa, and there was very significant difference in every type of dysplasia(P<0.01).3.The positive expression of p53 and CerbB-2 among all these syndromes of TCM hadsignificant difference (P<0.01), and they both had a significant stepwise crescendo tendencyin the following sequence, Disharmony between the Liver and Stomach, Damp Heat in theSpleen and Stomach, Deficiency of the Spleen and Stomach, Deficiency of Stomach Yin,Blood Stagnation of the Stomach.4.The positive expression of PCNA had not significant difference in every syndrome of TCM(p>0.05).5. Significant difference of the nuclear DNA content was found in all these syndromes ofTCM, and the regular sequence was Disharmony between the Liver and Stomach, Damp Heatin the Spleen and Stomach, Deficiency of the Spleen and Stomach, Deficiency of StomachYin, Blood Stagnation of the Stomach.Conclusions:l.The syndromes of TCM of chronic atrophic gastritis with dysplasia were on the basis ofpathologic histology and molecule biology reliably, and it might play partly a good guidancein treating this disease.2.Mild, moderate and severe dysplasia all had the possibility of carcinogenesis, and thepossibility increased with the increment of the severity of dysplasia in gastric mucosa.3. The worse of the syndromes of TCM of chronic atrophic gastritis with dysplasia increased by degrees, and the regular sequence was Disharmony between the Liver and Stomach, Damp Heat in the Spleen and Stomach, Deficiency of the Spleen and Stomach, Deficiency of Stomach Yin, Blood Stagnation of the Stomach.4. It had been approved that p53% CerbB-2 and the nuclear DNA content could be used as some important indexes for diagnosis and classification of chronic atrophic gastritis with dysplasia, and for guidance of treatment.5. The sensitivity of PCNA was higher, while its particularity was inadequate. It was unsuitable to be as the classification index, but it was helpful to diagnosis.