| Bronchial asthma (BA) refers to chronic allergic inflammation of airway caused mainly by EOS infliltration, which lead to airway obstruction and AHR. BA is a frequent allergic disorder. There are nearly 3 hundred million on the world, and with the increase of global industrializaton and pollution, the higher incidence and mortality of BA occurs which threat the health and living quality of the patients. In recent years, plenty of studies on clinical and experiments have shown that Acupoint Application (AA) has significant effect on BA, especially has less side effect and longer theraputic effect than west medicine. The article overviewed the etiology and Pathology, sydrome differentiation on BA of TCM doctors, summed up the developments of Acupoint Application in treating BA, outlined the realizaton on eidemiology, physiology, pathology and the latest development of basic reaserch (eg: the role of EOS on BA) in West Medicine, analyzed the limitation and puzzle of West Medicine in treating BA, prospected the studying direction in the future.ObjectiveWe established BA Model Guinea Pigs allergized with EA , chosed indexes correlated closely with chronic allergic inflammation of airway, such as EOS infiltration arid ultrastructure of bronchial epitheium, EOS apotosis, protein expression of Fas Fas-L and bcl-2 gene, cytokine(TNF, IL-2, TGF-β ) and adhesion molecule(ICAM-1, VCAM-1, L, E, P selectin), and detected their change after Acupoint Application. Through the research we can approach the Influence on and Mechanism of Airway Inflammation of BA treated with Acupoint Application comprehensively.Method40 Hartly Guinea-Pigs were devided medially into four groups: normal control group, BA model group, AA group and Dexamethasone group. EA sensitization mothod was used to establish the models of Guinea-Pigs with BA.The animals of AA group were treated by placing Chinese medicine at the point of Dazhui, Feishu and shenshu for 6 hours after depilation. The treatment was done once every other day. The animals of Dexamethasone group were given Dexamethasone 0. 5mg/kg once every other day by the method of intraperitoneal injectin. The two groups were treated 7 times simultaneously while there are no any treatment in control group and BA model group. After treatment we had the number of blood EOS counted, adopted the staining of HE to observe the EOS infliltration in bronchi and lung, used electron microscope to observe the ultrastructure of bronchial epitheium, adopted the method of TUNEL to detect the EOS apotosis, adopted immunohistochemical method to test the protein expressin of Fas , Fas-L and bcl-2 gene, TNF, IL-2, TGF~Pk ICAM-U VCAM-K Ln E> P selectin in bronchi and lung, adopted radioimmunity to test TNF, IL-2, TGF-Pi in blood and used euzymelinked immunosorbent assay to test ICAM-1> VCAM-K Ln E^ P selectin in blood. All data were shown in the formof *±s and analyze with one-way ANOVA , LSD and Dunnett T3 methods,, a = 0.05,Result1 The effect of AA on the number of EOS in blood and the EOS infliltration in bronchi and lung of BA Model Guinea-PigThe result showed: Comparing to control group, the number of EOS in blood and the EOS infiltration in bronchi and lung in BA model group increase obviously (P<0.01). Comparing to BA model group, the number of EOS in blood and the EOS infiltration in bronchi and lung in AA group and Dexamethasone group decreased obviously (P<0. 01). The EOS infiltration in bronchi and lung in AA group is obviously more than that in Dexamethasone group(P<0. 01), but there is no difference in the number of EOS in blood between the two groups(P>0. 05).2 The effect of AA on ultrastructure of bronchial epitheium of BA Model Guinea-PigThe result showed: in BA model group, we could see many destroyed, derangedand even necrotic cilia. Most of the microtubules in cilia were obstructed. Lots of EOS infiltrate among bronchial epithelial cells, which are mainly low-density EOS. Comparing to BA model group, the inflammatory destruction of cilia in AA group decreased obviously. There were more cilia that lined up better in order. Their obstruction, necrosis and EOS infiltration among them decreased. Comparing to BA model group, the inflammatory destruction of cilia in Dexamethasone group decreased obviously too. EOS was not seen but a few neutrohil could be seen.3 The effect of AA on EOS apotosis of BA Model Guinea-PigThe result showed: Comparing to control group, the rate of EOS apotosis in bronchi and lung in BA model group had no defference (P>0. 05). Comparing to BA model group, the rate of EOS apotosis in bronchi and lung in AA group and Dexamethasone group increased obviously (P<0.01). Comparing to Dexamethasone group, the rate of EOS EOS apotosis in AA model group decrease obviously (P<0. 01).4 The effect of AA on expression of Fas FasHL and bcl-2 gene of BA Model Guinea-PigThe result showed: Comparing to control group, the expression of Fas and Fas-L in bronchi and lung in BA model group had no defference (P>0.05). Comparing to BA model group, the expression of Fas and Fas~L in bronchi and lung in AA group and Dexamethasone group increased obviously (P<0.01). Comparing to Dexamethasone group, the expression of Fas and Fas-L in AA group decrease obviously (P<0.01).Comparing to control group, the expression of bcl-2 in bronchi and lung in BA model group had no defference (P>0. 05). Comparing to BA model group, the expression of bcl-2 only in AA group decreased obviously (P<0. 05), and it had no obviously defference (P>0. 05) in Dexamethasone group.5 The effect of AA on corrolative cytokine TNF, IL-2, TGF-Pl of BA Model Guinea-PigThe result showed: Comparing to control group, the level of cytokine TNF, IL-2. TGF- 3i of serum in BA model group increased obviously (P<0.01,0. 05 respectively). Comparing to BA modei group, the level of cytokine TNF, IL-2, TGF-ft in AA group and Dexamethasone group decreased obviously (P<0. 01,0. 05 respectively). There was no obviously defference between AA group and Dexamethasone group (P>0. 05).6 The effect of AA on corrolative adhesion molecule ICAM-K VCAM-K U E^ P selectin of BA Model Guinea-Pig6.1 The result of level of serum sICAM-K sVCAM-K E selectin .. P selectin and L selectin of BA Model Guinea-PigThe result showed: Comparing to control group, the level of serum sICAM-K sVCAM-K E selectin , P selectin and L selectin in BA model group increased obviously (P<0. 01). Comparing to BA model group, the level of serum sICAM-K sVCAM-K E selectin N P selectin and L selectin in AA group and Dexamethasone group decreased obviously (P<0. 01,). Comparing to Dexamethasone group, the level of serum sICAM-K sVCAM-1 in AA group increased obviously (P<0.05), wherease E selectin .. P selectin and L selectin had no obviously defference(P>0. 05).6.2 The result of expression of ICAM-K VCAM-K E selectin > P selectin and L selectin in bronchi and lung of BA Model Guinea-PigThe result showed: Comparing to control group, the expression of ICAM-K VCAM-K E selectin > P selectin and L selectin in bronchi and lung in BA model group increased obviously (P<0.01). Comparing to BA model group, the expression of ICAM-K VCAM-K E selectin -. P selectin and L selectin in AA group and Dexamethasone group decreased obviously (P<0. 01, 0. 05 respectively). Comparing to Dexamethasone group, the expression of VCAM-K P selectin in AA group increased obviously (P<0. 05), wherease ICAM-K E selecting L selectin had no obviously defference(P>0. 05).Conclusion1 AA therapy can decrease the number of EOS in blood and the EOS infliltration in bronchi and lung.2 AA therapy can relieve the inflammatory destruction of bronchial epithelial cells.3 AA therapy can promote EOS apotosis.4 AA therapy can improve the expression of Fas and Fas-L on EOS and restrain the expression of bcl-2.5 AA therapy can decrease serum TNF, IL-2, TGF-ft .6 AA therapy can decrease the expression of ICAM-K VCAM-K U En P selectin in bronchi and lung and the level of serum sICAM-K sVCAM-K E selectin > P selectin and L selectin.
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