| The experiment explored the mechanism and curative effect of Compound Biejia Ruangan Prescription (CBRP) to IPF by means of cellular molecular biology of IPF treated with CBRP. 180 male SD rats were randomly divided into six groups: sham operation group, model group, positive control group, high dose group, medium dose group and low dose group. There were 30 rats in each group. Their tracheas were exposed after being narcotized. Rats of every group were injected once with 0.3 ml bleomycin (BLM) physiological saline solution except that of sham operation group which was injected physiological saline solution. The rats were given the medicine at the same time of modeling. Rats of sham operation group and model group were poured with physiological saline solution into stomach. And the rest were poured with CBRP. 10 rats of each group were selected from every group on 7th day, 14th day and 28th day. Carotids were dissected after being narcotized. 4% paraformaldehyde was poured. 8 of them were made for light microscope specimen to fix. 2 rats were operated to open thorax and get lung tissue speedily. The latter was put into liquid nitrogen promptly to be frozen for hybridizing in situ , abstracting total RNA of MMP-2, determining RT-PCR and making electron microscope specimen. Light microscope specimens were made in regularly way. Immunohistochemiscal glass covers were dealt with DEPC for use. Transmission electron microscope specimens were made with routine method . Method and steps of material getting for ruthenium red staining electron microscope specimen were the same as that of transmission electron microscope specimens especially fixing with pre-fixer and post-fixer. Method of making ultra thin sections was the same as that of transmission electron microscope. Hybridization in situ: applying frozen slicing, patching, pre-processing of hybridization, RNA enzyme comparison processing, digesting with protein enzyme. MMP-2RT-PCR: firstly making primer designing, then abstracting total RNA, conducting reverse transcription, amplifying with PCR machine, determining with agarose gel electrophoresis. Results with HE staining under light microscope : Bronchus and alveolus of sham operation group of 7d, 14d and 28d were normal. Great inflammatory exudation and inflammatory cells were observed in model group of 7d. Fiber hyperplastic focuses were observed lying around alveolus interval and bronchiole. Exudation and pleura thickening were seen in positive control group that didn't exist in rest group. Except sham operation group quantity and structure of ATâ…¡ of every group were destroyed. There were inflammatory cells in alveolus. After 14d high, medium, low CBRP groups and positive control group showed various degrees fibrosis focus. Fiber hyperplastic focus was increased in model group. Consolidation area appeared. There were foam cells in alveolus. Next is positive control group in which consolidation area appeared around part of bronchiole. Inflammatory of every group reduced distinctly on 28d. A few inflammatory cells remained in part of area. Fibrosis of model group in alveolus interval and around bronchia was more obviously. Fiber appeared coil shape and glomerulus shape. The condition of positive control group was better. Fibrosis degree was not too high in alveolus interval. Fibrosis focus still appeared around bronchus. Inflammatory cells scattered in some alveolus and bronchiole. Pleura thickened. Conditions of high, medium and low CBRP groups became more distinctly. Fiber hyperplastic focus was less in alveolus interval and there weren't inflammatory cells in alveolus. Fibrosis degree was obviously lower than that of model group and positive control group. Among the 3 groups medium and low CBRP groups were more effective because few little fibrosis focus remained and structure of alveolus was restored closely to regular comparison group. The results showed CBRP had clear curative effect to IPF by reducing fiber piling and restraining forming of fibrosis. Mallory staining results: The results showed hyperplasia...
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