The Effects Of Dai Fan San On Liver Cell Memberane Of Estrogen-induced Intrahepatic Cholestasis In Rats

Objective Intrahepatic cholestasis(IC) is the common fundational pathologic changes. Recently the sick rat of IC is rising caused by estrogen with more and more wemen taking estrogen. The study in pathogenic mechanism of IC have penetrated into the molecular level in forenge, but there was not any effective treatment pharmaceuticals besides ursodeoxy cholic acide(UDCA). In my country, the study of IC is in the class of lower primary but the Chinese medicine has showed the primary effective and some superiority and speciality.The main study in Chinese medicine to IC focused at clinical and was limited to observating changes of clinical symptoms but was short of the definite experiment's foundation. The clinical studies proved that Chinese medicine Dai Fan San(made up by Ming Fan and Qing Dai) was a sort of effective treatment to IC caused by virus hepatitis ,deseases of biliary tract and pregnancy et al, but it was short of the experiment's foundation. In accordance with the progress, the aim of our study was to probe into the pharmaccoligy mechanism of Dai Fan San and supply the science basis and establish the base of seeking for active material medica to IC ,through observing the effect of Dai Fan San and its components to rat's liver function , liver histology, liver cell ultrastructure, hypatocyte memberane fluidity and Na⁺-K⁺-ATPase, Mg⁺⁺-ATPase of IC induced by estradio benzate(EB). Methods Use EB to replicate cholestasis model and observe the effect of Dai Fan San and its components on the liver function (TBA,Tbil,ALT and ALP), liver histology, liver cell ultrastructure, hypatocyte sub-membrane fluidity(cluding hypoctocyte sinus membrane and bile capillaries membrane ) and Na⁺-K⁺-ATPase, Mg⁺⁺-ATPase in liver homogenate and hypoctocyte sinus membrane of rat's IC induced by EB.Results The results of the experiment show that the rat's liver function (TBA,Tbil,ALT and ALP) all go up distinctly (PO.05) ,but Tch go down obviously (P<0.05); ALP in hepatic even cytoplasm and cholangiole membrane go up prominenty (P<0.05); the activity of Na⁺-K⁺-ATPase, Mg⁺⁺-ATPase all go down obviously (P<0.05); Dai Fan San and its components filled by mouth.can always hider...