MAPK Signal Transduction Significance In Hepatocellular Carcinoma

Hepatocellular carcinoma (HCC) is one of the most common cancer in the world.The risk factors of HCC include hepatitis B virus (HBV) ,hepatitis C virus (HCV), aflatoxin B1 (AFB1) and genetic factors and so on. These risk factors of HCC can fall into two subgroup,one is environmental factors and the other is inherited factors. The mechanism of tumorigenesis of these risk factors are either activated the oncogenes or inactivated the tumor suppressor genes, or disturb control of cell cycle, or interfere with the normal regulation of cell differentiation, or abnormal apoptosis and signal transduction and so on; The data about signal transduction pathway of HCC show that a lot of signal transduction pathway participate in the hepatocarcinogenesis. Mitogen-activated protein kinase (MAPK) pathway is a important pathway that the signal transfer from surface of the cell to the nuclear. The data from other researchers show that the MAPK pathway play an important role in many malignant tumors but it is unclear in the HCC. Our study is in order to explore the significance of MAPK pathway in HCC and potential offer a new strategy for prevention and treatment of HCC.The mRNA of Extracellular regulated protein kinase1/2, c-Jun N-terminal kinase (JNK1) and p38 of the HCC tissues, HCC-adjacent tissues, and biopsy liver tissues were detected by RT-PCR method. The phosphated protein p-ERK1/2 and p-JNK1 were detected with western blotting and immunohistochemical analysis. In addition, the HBxAg and the mutation of the p53 gene had been detected too.