Biosynthesis And Biotransformation Of Securinega Alkaloids

Securinine showed effective CNS-stimulating impacts as a kind of stereospecific antagonist of GABA. It has been used as an important clinic drug or common tool medicine. Recent researches indicated securinine was helpful to improve the cognitive deficits and neurodegeneration, and may be a potential medicine for the treatment of Alzheimer's disease. Due to their attractive biological activity, the studies on the securinega alkaloids were increasing in recent years. Securinine is a sort of compounds, which are difficult to be chemically synthesized or derived for its rigid and chiral structure. Importantly, it is quite unusual that the respective plants of Securinega suffruticosa growing in various locations are able to produce different enantiomers. Due to no enantiomers were detected together in one single plant of S. suffruticosa so far, S. suffruticosa is to be a good plant material to investigate the biosynthesis of chiral compounds. Therefore, It is very worthwhile to do researches on biosynthesis and biotransformation of securinine.1. Callus cultures of Securinega suffruticosa and the accumulation of securinine in the callusThree callus cultures of S. suffruticosa were induced from original plants collected from three locations: Xinglong county in Hebei province, Miyun county in Beijing, and Shangfang Mountain of Fangshan district in Beijing. HPLC conditions for analysis of securinine in the callus were optimized. Securinine with dextro rotation was testified to be accumulated in the callus by different spectral methods. A simple method for enantiomeric discrimination of (± )securinine using the chiral solvating agent (S)-(-)-N-Phthalylalanine was established. Time course studies on...