Biotransformation Of Three Active Sesquiterpenes

It was firstly reported that Cordyceps militaris could be used to transform the nature products. The fermentation medium of C. militaris as a biotransformation system was optimized by systemic analysis of the flask fermentation included sugar (6%), yeast extract (0.4%), MgSO₄ (0.05%); CaCl₂(0.01%) and KH-2PO₄(0.1%). The optimum fermentation conditions were 10% of inoculated volume in fermentation condition of fermenting temperature 24 °C, the initial PH value (6.0) and small light needed. At the same time, we studied the chemical constituents in the liquid culture of Cordyceps militaris by various chromatographic methods. 32 compounds were obtained from the liquid culture and the structures of 29 compounds were identified as P-sitosterol (C-l); 2-ethyl-3-hydroxy, 4H-pyran-4-one(C-2); 7, 8-dimethyl-isoalloxazine (C-3); 6-isobutyl-pyrorolo [1,2a] hexahydropyrazine-1, 4-dione (C-4); 6-benzyl-pyrrolo [1,2a] hexahydropyrazine-1, 4- dione (C-5); 6-isopropyl-pyrrolo [1,2a] hexahydropyrazine-1, 4-dione (C-6); 3, 7, 8-trimethyl -isoalloxazine (C-7); 1-formyl-benzpyrazole (C-8); 2H-pyran-2-one, 3-ethyl-4-hydroxy-6-methyl (C-9) ; daucosterol (C-10) ; 6-methyl-pyrorolo [1, 2a] hexahydropyrazine-1, 4-dione (C-11); l-methyl-6-isobutyl-2, 5-piperazine-dione (C-12); l-isopropyl-6-isobutyl-2, 5-piperazine-dione (C-13); D-mannitol (C-14); succinic acid (C-15); uracil (C-16); 2-methyl- 3-hydroxy- pyrolidine (C-17); thymine (C-18); l-hydroxymethyl-6-isobutyl-2, 5-piperazine-dione (C-19); cordycepin (C-20); adenosine (C-21); 3, 6-di-(p-hydroxybenzyl)- 2, 5-piperazine-dione (C-22); succinamide, N, N-bis- [2-(3-indol) -ethyl] (C-23); flazin (C-24); 3- acetamino- 6-isobutyl -2, 5-piperazine-dione (C-25); 3, 6- bis-(3-methyl-indole)-2, 5- piperazine -dione (C-26); guanosin (C-27); 5-isobutyl -3, 6-dioxo-2- piperazinepanoic acid (C-28); ergosta-7, 22-diene-3β, 5, 6-triol (C-29). Among them, 3-acetamino - 6-isobutyl-2, 5-piperazine-dione (C-25) is a new compound. The compounds C-8,...