| Three hybridomas producing monoclonal antibodies (McAb) against recombinant human granulocyte colony-stimulating factor (rhG-CSF) have been established by fusing mouse myeloma cell line SP 2/0 with spleen cells from a BALB/c mouse immunized with rhG-CSF.Aseites are obtained from BALA/c mice by injecting the hybridoma cells of AG2D4-B4 substrain intraperitoneally. The antibody is purified either by caprylic acid-ammonium sulfate method or by euglobulin precipitation. The caprylic acid-ammonium sulfate method is superior to euglobulin precipitation in purification and recovery of IgG. The McAb AG2D4-B4 is determined as IgG3 subclass with molecular weight of about 281,500 dalton. It is proved by Western Blot that the McAb AG2D4-B4 can react specifically with rhG-CSF.The application of this McAb in diagnosis and research is discussed.
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