The Effects Of Cold Preservation On Intrahepatic Biliary Microcirculation Following Rat Liver Transplantion

With the development fo technolgy, liver transplantation has become the only definitive method of therapy for patients with end-stage liver diseases. However, the morbidity of ischemic-type biliary lesion(ITBL) after liver transplantation has kept in high level for long time. The ITBL seriously impacts survival rate and life guality of the recipients with liver transplantation. Many specialists have been payed more attention to the study on ITBL. However the cause of ITBL is still unclear. It is believed that Biliary epithelial cell(BEC) is the primary target of cold preservation /reperfusion injury (CP/RI), as BEC is more sensitive to this kind of injury than hepatocyte. After long duration of cold preservation of allografts, even liver funtion can keep good states following transplantation, but the incidence of ITBL is greatly increased. Cold preservation will cause biliary epithelium necrosis and fibrosis , and followed by biliary sludge and stricture, thus causes ITBL.It has been found that the obstruction of hepatic tissue,s microcirculation play an important role in liver CP/RI. But people have neglected the CP/RI to biliary microcirculation presently, we don't know whether cold preservation/reperfusion will cause the obstruction of biliary microcirculation, The relationship among the extent of diliary microcirculation vascular endothelial cell injury, microcirculation obstruction and biliary injury, is unclear. In clinical liver transplantation, the system of biliary circulation flushed deficiently and hepatic artery obstruction following transplantation will cause serious biliary injury and ITBL .Allograft must experences cold preservation and reperfusion in liver transplantation. How to prevent the ITBL caused by biliary CP/RI is a hotspot studying of liver transplantation. It has been found that GSNO may be a kind of perfect NO donor. GSNO can slowly and continously releases NO in body. So it can steadily improve microcirculation, and decrease tissue CP/RI. However, we don't know whether GSNO will improve biliary microcirculation, and decrease biliary injury caused by cold preservation...