Study On The Association Of Mycoplasma Genitalium And Ureaplasma Urealyticum In Men With Acute Nongonococcal Urethritis

Objectives To investigate the infection or colonization of Mycoplasma genitalium and Ureaplasma urealyticum in different male populations, to demonstrate the association of M. genitalium and U. urealyticum with nongonococcal urethritis (NGU) respectively, to assess the association between the ureaplasma biovars (or genotypes) 1 and 2 and urethritis, and finally to facilitate further aetiological study to provide the evidence for STD control strategy making.Methods In the first part of this study, four different male populations, namely: NGU patients(Gl), non-NGU subjects attending STD clinic(G2), men who have sex with men participating a health education program(G3) and healthy volunteers(G4) were recruited randomizedly. Nested PCR and culture were used to detect U. urealyticum, Nested PCR and PCR product sequencing were applied to detect M. genitalium. The demographic, clinical, sexual behavioral characteristics in relation to the infection or colonization of M. genitalium and U. urealyticum were analyzed by using SPSS 12.0. Consecutively in the second part of this study, G1 and G2 samples were used as case-controls to study the association of M genitalium and NGU, meanwhile, the PCR and nucleic acid hybridization assay for the detection of C. trachomatis and N. gonorrheae were performed. This assay was also used to monitor for PCR inhibition in all samples by use of the internal control provided in the kit. Finally in the third part, three pairs of primers were used to determine the genotypes of U. urealyticum, and the genotypes correlating with NGU were analyzed.Results The prevelence rate of M. genitalium in four study populations was 25.0% (25/100), 6.4% (6/94), 5.5%(6/110) and 0 respectively. There was significant difference between each group except G2-G3 with a p value of 0.80. By multivariate analyses, controlling for new sexual partner, urethral discharge and condom use in previous 3 months, only present urethral discharge was associated with M. genitalium (OR=6.754, 95% CI 1.833-24.893). The direct...