The Mechanism Of Overexpression Smad7 Improving Peritoneal Transport Function In Peritoneal Fibrosis Rat Model

Part 1: Effects of overexpression Smad7 on the expression of VEGF and vascular proliferation in a rat peritoneal fibrosis modelChapters 1: The effects and significance of different glucose concentrations on the expression of VEGF and vascular proliferation in the peritoneal membrane of dialysis ratObjective: To investigate the effects of different concentrations glucose on the expression of VEGF (vascular endothelial growth factor) and vascular proliferation in the peritoneal membrane of dialysis rat, and to reveal the mechanism of peritoneal transport.Methods: SD rat were randomly divided into four group.Group A is normal control group; Group B:dayly received intraperitoneal injection of 1.5% glucose dialysis fluid for four weeks; Group C: dayly received intraperitoneal injection of 2.5% glucose dialysis fluid for four weeks; Group D: dayly received intraperitoneal injection of 4.25% glucose dialysis fluid for four weeks. RT-PCR,Western blot, immunofluorescence and immunohistochemistry technology were employed to detect the expression of VEGF and number of vascular vessels, and the amount of ultrafiltration in four groups was also measured.Results: The expression of VEGF was increased gradually with the increasing of glucose concentration, and Group D was most significant of all four group. RT-PCR reveal that there was a 9-fold up-regulation of VEGF mRNA expression in group D compared with that of group A (48.67±13.61 vs 5.33 ± 2.3; P < 0.001); And it accompanied with new vascular vessels increased, Immunohistochemistry of CD31 show that the...