Effects Of Different Concentrations Of Peritoneal Dialysis Fluid On The Expressions Of VEGF And Neoangiogenesis Of CAPD Rat Peritoneal

Objections: In the present study, we comprehend the changes of expression ofMCP-1 and VEGF of PMC in short-term period through the rat CAPD models, andthen to investigate the effects of PDF on MCP-1 and VEGF producted by PMC innon-infection situations and the associations with CAPD related peritoneal sclerosis.Methods: Experiments were performed on 50 adult male Sprague-Dawley ratsweighing Between280-350g. Rats were randomized into 5 groups randomly anddialyzed with Various solution as follows:(1)no solution(control group, n=10), (2)1.5% Dianeal solution(1.5%PD group,n=10) (3) 2.5% Dianeal solution(2.5%PDgroup,n=10), (4)4.25% Dianeal solution(4.25%PD group,n=10), (5) 0.9% Salinesolution (NS group, n=10), on the 29th day, after a 4-hour dwell, the rats were put todeath, dialysate samples and serum were collected to measure the MCP-1 VEGFproteinlevels using the ELISA kit, parietal peritoneum tissue were taken to determinethe MCP-1 VEGF expression by IHC.Results: The MCP-1 and VEGF protein expression in parietal peritoneum tissuewere significantly increased in 4.25%PD group 1.5%PD group and 2.5%PD groupcompared with control group (P＜0.05). The MCP-1 and VEGF protein expression in4.25%PD group were higher than that of 1.5%PD group and 2.5%PD group,but thedifferences between 4.25%PD group with 2.5%PD group, 1.5%PD group with2.5%PD group had no statistic significance (P＞0.05), while the level In NS groupwere significantly increased compared with the control group (P＜0.05). there were noMCP-1 and VEGF protein expression in control group.The MCP-1 and VEGF protein levels of PDF were increased in4.25%group comparedwith that of control group 1.5% and 2.5% group, and the difference had statisticsignificance (P＜0.05), while the values in NS control group 1.5% and 2.5% group were significantly increased compared with control group (P＜0.05). but thedifferences between 1.5%PD group with 2.5%PD group had no statistic significance(P＞0.05).The MCP-1 and VEGF protein levels of serum in the whole 5 groups showed nosignificant difference (P＞0.05).neovascularization were more prominent in the 4.25%PD group than the controlgroup 1.5% and 2.5% groups, while the levels in NS group were significantlyincreased compared with the control group (P＜0.05). The neovascularization of2.5%group and 1.5%group compared to the control group were significantlyincreased (P＜0.05). but the differences between 1.5%PD group with 2.5%PD grouphad no statistic significance (P＞0.05).The MCP-1 and VEGF protein expression in parietal peritoneum tissue had directcorrelation (r=0.850,P＜0.01). We also do the correlation analysis between VEGF andMCP-1 protein levels of PDF, and found positive correlation (r=0.810,P＜0.01).Conclusion: PDF could induced the increase of MCP-1 and VEGF proteinexpression of CAPD rat PMC, and the increase followed by the increase of glucoseconcentrations of PDF.Accumulation of VEGF was associated with the increase expression of MCP-1. Theexpression of these cytokine were not concerned with general circulation. But howthey effected each other still need further studys.The most important factor of peritoneum neoangiogenesis is the merocrine secretionof VEGF.Hyper glucose dialysate in CAPD rats may lead to the peritoneum ultrafiltrationfailure and neoangiogenesis by promoting the expression of MCP-1 and VEGF, andimpair the mesothelial cells.