The Role Of HMGB1 In Rheumatoid Arthritis And Experimental Arthritis And Studies On Intervention Of ～(99)Tc-MDP

Background: Rheumatoid arthritis(RA) is a systemic inflammatory autoimmune disease, with high rate of disability. Although the pathogenesis and therapy strategy of RA have been studied extensively for many years, but its etiopathogenisis is not clear and the therapeutic outcome is not satisfied. Joint destruction occur in about half patients within two years. It is one of major diseases which cause loss of labor force and disability in China. In recent years, it's found that high mobility group box protein 1 (HMGB-1) involved in the pathogenesis of RA. Nuclear HMGB-1 expression was accompanied by a cytoplasmic staining in many mononuclear cells, with a macrophage-like appearance and an extracellular matrix deposition in adjuvant-induced arthritis rats. HMGB-1 was more strongly expressed in synovial fluid(SF) of RA patients than in that of OA patients. There are miscellaneous results about whether the expression of HMGB1 increases in the serum of RA patients. The further studies are needed in the expression of HMGB-1 in peripheral blood mononuclear cells(PBMCs) and the serum, the correlation of the expression of the HMGB1 in serum with the clinical manifestations, the relationship between HMGB1 and joint damage, and the effect of common antirheumatic drugs on the expression of HMGB1.Part â…  The expression of HMGB-1 in the peripheral blood and synovial fluid of patients with rheumatoid arthritis and its clinical significanceObjective: To investigate the expression of HMGB-1 in PBMCs, serum and synovial fluid of RA patients and to analyze correlation with clinical features such as erythrocyte sedimentation rate (ESR) , C-reactive protein (CRP), rheumatoid arthritis (RF), numbers of tender joints and swollen joints and radiographic scores.Methods: HMGB-1 mRNA was detected by reverse transcription chain reaction (RT-PCR) in PBMC and HMGBl protein in PBMC and serum by Western blot of 38 patients with active RA, 24 patients with inactive RA and 20 healthy controls. HMGB-1 protein in synovial fluid was detected by Western...