The Expression Of Exogenous HEGF And HbFGF After Transfected Into Human Keratinocytes And The Biological Effects Of The Active Materials Secreted By The Two CDNA-modified Keratinocytes On The Normal Human Keratinocytes, Dermal Fibroblasts And Dermal Micro

The study on the relationship between to peptide growth factors and wound healing, has revealed a potential way to the wound healing clinic. There have been two limits to the applications of the peptide growth factors in fact; of which is the growth factors need keeping in a effective level in the wounded tissues, the other is one growth factor need the presentation of an other suitable growth factor at the same time when it functions well. With the quicke development of molecular biochemistry, several cDNAs including some encoding the molecules of growth factor have been successfully transfected into the human keratinocytes and expressed the corresponding proteins which secreted then by the gene-modified keratinocytes. This showed an attractive new way to the people.To investigate the potential of the gene-modified keratinocytes which could release the interesting growth factors as a vector, two cooperative growth factors: hEGF and hbFGF, hEGF cDNA and hbFGF cDNA which encode were colonized into the eukaryotic expression vector: pcDNA₃-neo, so pcDNA₃-hEGF and pcDNA₃-hbFGF were constructed. Lipofectamine was employed to transfer the pcDNA₃-hEGF and pcDNA₃-hbFGF into the primary cultivated human keratinocyets. The gene-transfected keratinocytes were then purified with G418. pcDNA₃-hEGF and pcDNA₃-hbFGF were detected in the gene-transfected keratinocytes by in situ hybridization using specific probe for...