Effect Of Modified Taohong Siwu Decoction On The Secretion Of Endotheline-1 And Basic Fibroblast Growth Factor Of Keratinocytes

Background and ObjectiveVitiligo is a kind of disease characterized by the depigmentation of the skin and hair because of acquired melanocytes damage. At present, the pathogenesis of vitiligo remains unclear, but many domestic and abroad researches have proved that vitiligo may be related with heredity, immunity and nerval elements. Of late years, more and more studies find that the cytokines participate in the development of vitiligo. There are similarities between keratinocytes and melanocytes, and the cytokines secreted by keratinocytes play certain roles in the survival and activation of melanocytes. Human keratinocytes have been shown to synthesize and secrete endothelin-l(ET-l) and basic fibroblast growth factor (bFGF) in culture. Recently it has been shown that ET-1 is able to stimulate melanocytes proliferation and tyrosinase activity via a receptor-mediated signal transduction pathway, and bFGF is a potent mitogen for melanocytes. For these reasons, it is important to study the relationship between ET-1 and bFGF and vitiligo. As yet we haven't seen any published article on the effect of Chinese traditional medicine on keratinocytes secreting ET-1 and bFGF.In order to explore the mechanism of Chinese traditional medicine treating vitiligo. we investigate the effect of Modified Taohong Decoction (Pikang oral liquid)on keratinocytes secreting ET-1 and bFGF. Materials and Methods1. MaterialsHuman Keratinocytes: the normal child foreskin was collected, and keratinocytes were separated and cultured. The fourth era cells were investigated.Chinese traditional medicine: Modified Taohong Siwu Decoction (Pikang oral liquid), which contains radix astragali, dry radix ginseng, radix angelicae sinensis, radix salviae militiorrhizae, radix rehmanniae, flos carthami, semen persicae, radix paeoniae rubra, rhizoma chuanxiong, fructus ligustri lucidi, herba hyperici and so on, provided by the pharmaceutical department of our hospital. 10mL Pikang oral liquid corresponds to 12.2 gram of raw.2. MethodsThe effects of Chinese traditional medicine on keratinocyte proliferation were measured by MTT method.The effects of Chinese traditional medicine on the ET-1 and bFGF secretion of keratinocytes were measured by ELISA technique using commercially available ELISA Kits according to the manufacturer's instructions.3. Statistical analysisAll the data were analysized using SPSS 10.0 statistical software. Student's Mest was used to analysis the statistical differences between Chinese traditional .medicine and control.Results1. The cells proliferation indexs were significantly higher in high concentration Pikang oral liquids (4.27, 5.49, 6.1mg/mL) than in of blank controls(P<0.05), and no significant differences were observed between low concentration Pikang oral liquids(1.22, 1.83, 3.05mg/mL) and blank controls (P>0.05) .2. ET-1 secreted by keratinocytes were significantly greater in both high concentration(5.49mg/mL) and low concentration (1.83mg/mL) Pikang oral liquid than that in blank controls(,P<0.01), and no significant differences were observed between different concentration Pikang oral liquids and blank controls in bFGF secreted by keratinocytes.(T>0.05) .Conclutions1. High concentration of Pikang oral liquid can enhance the proliferation of keratinocytes, and low concentration of Pikang oral liquid have no effect on the proliferation of keratinocytes.2. Pikang oral liquid can stimulate keratinocytes to secrete ET-1, and have no effect on the secretion of bFGF.3. The results suggest that the therapeutic effect of Pikang oral liquid on vitiligo may be related to promoting the ET-1 secretion of keratinocytes and stimulating the proliferation and melanogenesis of melanocytes.