The Ultrasonographic Observations On The Proliferating Activity And Invasiveness Of The VX2 Tumor In The Rabbit Liver

Objective: There were three parts in the experimental study, which included" Dynamic observation on the VX2 tumor in the rabbit liver with ultrasonography", "The meanings of US in describing the invasiveness of the VX2 tumor in the rabbit liver", and "The relationship between the vascularity indicated by US and the cellular proliferation of the VX2 tumor in the rabbit liver". As it is impossible to diagnose the hepatic carcinomas at the cellular-level for ultrasonography at present, the purposes of the study were attempted to evaluate the significance of the routine parameters of US in inferring the malignantly biologic characteristics including tumor invasiveness of the hepatic carcinomas, and to provide some preclinical animal experimental references for early diagnosing hepatic carcinoma by observing and analyzing the ultrasonographic progression of the liver cancer less than 3.0 cm in diameter in vivo animal model, and by finding out the correlation between the US parameters and the proliferating activity in the animal liver carcinomas. Material and methods: The basic procedures included setting up the animal models of the VX2 tumor in the rabbit liver, making clear the feasibility of the animal model for the US observation on hepatic carcinomas, selecting suitable parameters for US depicting the hepatic tumors, proving the US detection of the tumor by using X-ray DSA, MRI and SPECT, and comparing the US image of the tumor with the results of histopathologic section, DNA analysis and PCNA LI of the tumors. Results: The results showed that the mean size in diameter of the tumor had a direct proportion to the tumor implanted days while the intratumor echoic patterns of the tumor could be classified into hyper-, iso-, and hypoechoic types into which the tumors could change each other as the masses grew. The...