Mechanisms Of UV-induced Acute Damage Of Human Dermal Fibroblasts And The Prevention

The sun gives off ultraviolet radiation that we divide into categories (UVC - 200 to 280 nm, UVB - 280 to 320 nm, UVA - 320 to 400 nm) based on the wavelength. The skin is the first barrier of human body against UV damage. UVC radiation is almost completely absorbed by the ozone layer and does not affect the skin. Both UVA and UVB radiation is proven to be the most important facts to lead to an actue inflammation, photoaging and carcinogenesis. It has been found that UV exposure induced human skin keratinocytes and fibroblasts releasing pro-inflammatory factors which play major roles in initiation and activation of inflammatory processes in acute inflammation of the skin.In this study, we investigated the effects of ultraviolet on the acute damage of skin fibroblasts and the protective effects of mizolastine on UV exposed skin dermal fibroblasts; then, we compared the proteins expressed by normal human dermal fibroblasts and cells after UV exposure using proteomic techniques, to learn the mechanisms of acute skin damage induced by UV irradiation, and to search UV-relevanted proteins in fibroblasts.1. The mechanisms of UV-induced acute damage of skin fibroblasts and the action by mizolastine against UV-mediated inflammatory leukotriene B4 production.Certain amounts of ultraviolet irradiation may result in a sustained inflammatory reaction of the skin (i.e. sunburn), characterized by redness, swelling and pain, both in human and experimental animals. Previous studies showed that leukotriene B4 (LTB4)might play important roles in UVB irradiation-related skin injury. LTB4 is a mediator of inflammation generated through 5-lipoxygenase(5-LOX) pathway, with potent...