A Study On Germplasm Of Panax Ginseng And Its DNA Fingerprinting

Panax ginseng is one of the most medicinally important plant in China, and its yield and quality are affected by its germplasm to a great extent. However, owing to a special growing condition and a long reproductive cycle, the germplasm of ginseng, particularly wild mountain ginseng was hardly studied before. In the present dissertation, the resources of P. ginseng, wild mountain ginseng and five garden ginseng landing races, were studied comprehensively using DNA fingerprinting, a new molecular biology techinique. 92 collections of germplasm were used in the RAPD and AFLP analyses, and more than 600 sites in the genome were detected . In addition, nuclear ribosomal DNA internal transcribed spacers (ITS) of four wild mountain ginseng individuals were sequenced. Moreover , the seed - coat of five garden ginseng landing races was observed by scanning electron microscope (SEM) . The genetic characteristics of ginseng germplasm and their relationships revealed in our experiment would be beneficial to cultivation, breeding and conservation of ginseng. Our main points are as follows:1. The genetic variation of five landing races of Panax ginseng, i. e. , Damaya ( DMY ) , Ermaya ( EMY ) , changbo ( CB ) , Yuanbangyuanlu...