Loss Of Heterozygosity Analysis Of Chromosome 9p13-23 In Microdissected Laryngeal Squamous Cell Carcinoma

Loss of heterozygosity (LOH) studies indicate that allelic loss associated with the development of HNSCC occurs most frequently at 9p21-22. However, the target of chromosome 9p21 -22 loss has been the source of significant debate. A putative tumor suppressor gene, p16/CDKN2, has been identified at the 9p21 location, but genetic alterations of p16/CDKN2 located in this region are unusual. The gene encoding p16 mayn't be the primary target of LOH at 9p21-22 in HNSCC, implicating the existence of (an) additional tumor suppressor genes at this locus involved in the pathogenesis of HNSCC. To further refine the extent of allelic loss on chromosome 9p21-22 will be helpful to find other putative tumor suppressor genes at this locus.In this current study, the sections of paraffin-embedded tumor tissues were microdissected to enrich for neoplastic cells. To further investigate the extent and significance of the loss of the chromosome 9p21-23 region in laryngeal squamous cell carcinoma, we performed detailed mapping at this region in a set of 42 primary laryngeal squamous cell carcinomas for the hot spots of LOH using 13 highly polymorphic microsatellite markers. We also used the x~2 test or Fisher's exact test to compare the incidence of LOH in...