G-CSF Induces Stem Cell Mobilization By Interaction Of SDF-1/CXCR4 And MMP-9

Mobilization of hematopoietic stem and progenitor cells from the bone marrow into the circulation by repetitive, daily stimulation with Granulocyte colony-stimulating factor (G-CSF) is widely used for clinical transplantation. Although adequate CD34~+ cells were collected for a majority of patients by G-CSF alone, or in combination with myelosuppressive chemotherapy, some patients and also healthy donors are still poor mobilizers. Therefore, It is necessary to explore the mechanism of stem cell mobilization. It not only contributes to understand the changes in BM microenvironment during the process of mobilization, but also help us develop optimal strategies including novel mobilizing agents for poor mobilizers. In addition, it has been demonstrated that stem cell homing and release or mobilization are mirror images utilizing a similar mechanism, investigation of SDF-1/CXCR4 interactions in stem cell mobilization will help us understand stem cell homing to target organs as well.Recent studies have focus on close relation between SDF-1/CXCR4 and stem cell mobilization, which might be the ultimate pathway in modulation. At present, a majority of studies are limited to comparison of the expression levels of SDF-1/CXCR4 in steady-state BM and mobilized PB. Few studies were focused on SDF-1/CXCR4 in BM microenviroment after G-CSF injection. Therefore, G-CSF mobiliezed healthy donors were included in our...