Studies On The Secondary Metabolites Of Marine Microorganisms

Over 400 marine microorganism strains were collected and isolated from sea area all over the world. With morphological change of Pyricularia oryzae as active index, 98 active strains of them were screened preliminarily, 6 strains of which were found to have better activities. We studied on the chemical constituents of the fermentation and/or mycelia of these six strains by all kinds of chromatographical techniques (such as silica gel chromatography. sephadex LH-20, PTLC and HPLC etc.) with bioactivity-guided fractionation. 1(?) Compounds were isolated from strain 1b-1 (Aspergillus versicolor) and determined by means of physicochemical evidences and spectral methods. These 14 compounds were identified as sterigmatocystin (a-I), 6-methoxysterigmatocystin (a-II). averufin (a-III) 1,6-dimethoxylversicolorin B (a-IV), p-benzyloxy-phenol (a-V), tyrosine (a-VI), ergosta-5, 7, 22-triene-3β -ol (a-VII), 5α, 8α-epidioxy ergosta-6, 22-diene-3β-ol (a-VIII), amber acid (a-IX), carbamide (a-X), uracil (a-XI), mannitol (a-XII). 9 Compounds were isolated from strain SH-3 (Marinomonas sp.), and 7 of which were elucicated by use of physicochemical evidences and spectral methods. These 7 compounds were identified as pyrrolopiperazine-2, 5-dione (b-I), 3-methyl piperazine-2, 5-dione (b-II), 3-methyl-pyrro(?)piperazine-2, 5-dione (b-III), phenylacetic acid (b-IV), tyramine (b-V). carbamide (b-VI), 3-isopropyl-pyrrolopiperazine-2, 5-dione (b-VII). 12 Compounds were isolated from s(?)rain Z16-1 (Bacillus sp.), and 10 of which were elucidated by using physicochemical evidences and spectral methods. These 10 compounds were identified as pyrrolopiperazine-2, 5-dione (c-I), 3-methylpiperazine-2, 5-dione (c-II). 3-hydroxymethyl-8—hydroxyl pyrrolopiperazine -2,5-dione (c-III), thymine (c-IV), 3 -isobutyl — 6—se(?)butyl-piperazine-2, 5-dione (c-V), 3-isobutyl-pyrrolopiperazine-2, 5-dione (c-VI), tryptophan (c-VII), maricyclocin (c-VIII) ,β-sitosterol (c-IX), 3β-hydroxxy-cholesta -5- ene (c-X). 1(?) Compounds were isolated from strain Z11-1(Alternaria sp.), and 7 of which were elucidate(?) by use of physicochemical evidences and spectral methods. These 7 compounds v. re identified as (2S, 3S, 4R, 16E)-1,4-epoxy-2-[(2R)-2-hydroxy hexadecanoyla(?)no]-16- hexacosene-1, 3, 4-triol (d-I), (2S, 3S, 4R.