Research On RECK Regulation And UFA Effecting In Lymphatic Metastasis Of Tongue Squamous Cell Carcinoma

Objectives: Lymphatic metastasis of tongue squamous cell carcinoma is a major aspect of oral malignant tumor researches. MMPs family is considered to play a important role in tumor progression and metastasis, while RECK gene (a new MMPs inhibitor) can interfere activities of MMp-2 and MMp-9 to block tumor cell metastasis, and they compose the regular system of metastasis. We base this study on the clinical patho-detection and animal model of lymphatic metastasis, aiming to explore the relationship between the regulation of RECK gene with MMP-2 and MMP-9, elucidate the effect, clinicopathological significance and regular mechanism of RECK gene in lymphatic metastasis of tongue squamous cell carcinoma. Isatis tinctoria Lon can repress metastasis of lingual squamous cell carcinoma. The aim of next research is to investigate the effects of anti-invasion and anti-metastasis by unsaturated fatty acids from Isatis tinctoria Lon, and the mechanism was explored in vitro and in vivo.Methods: Experiment was divided into two parts, in the first part, lymphatic metastatic animal model of tongue squamous carcinoma was established and clinical samples from 59 patients with tongue squamous carcinoma were collected. Immunohistochemical staining of RECK ,MMP-2 , MMP-9 were...