Expression And Clinical Significance Of RECK Gene And Matrix Metalloproteinase-2, 9 In Bladder Cancer

Objective: To investigate the relationship between the expression RECK gene andmatrix metalloproteinase-2,9 (MMP-2,9) and invision or metastasis of transitionalcell carcinoma (TCC).Methods: RECK and MMP-2,9 gene expressing levels in 43 pairs of TCC tissuesand nontumorous bladder tissues were measured by semiquantitatively reversetranscription polymerase chain reaction (RT-PCR).Results: RECK messenger RNA (mRNA) expression was present in 40 of 43 (93%)nontumorous bladder tissues, whereas it was seen in 19 of 43 (44%) of resected TCCs.The expression level of RECKmRNA in nontumorous bladder tissues (0.2816±0.0588)was also obviously higher than in bladder cancers (0.1799±0.0419)(P＜0.05). Norevelent associations level of RECK expression with tumor cell grade and clinicalstage was found. MMP-2 mRNA expression was present in 33 of 43 (77%) resectedTCCs, whereas it was seen in 9 of 43 (21%) of nontumorous bladder tissues.MMP-9 mRNA expression was present in 23 of 43 (53%) resected TCCs, whereas itwas seen in 11 of 43 (26%) of nontumorous bladder tissues. The level of MMP-2,9expression in tumors tissue specimens(0.9291±0.2945, 0.7602±0.3230) wereobviously higher than adjacent normal bladder tissue(0.5878±0.1103, 0.5275±0.1138).(P＜0.05) The level of MMP-2,9 mRNA expression correlated significantly withtumor cell grade and clinical stage. In addition, the difference correlated with genderand age were no statistical significance in all genes. (P＞0.05).Conclusions: These results are in agreement with the nontion of RECK being antumor-supressor gene. Expression of MMP-2,9 correlates closely with the invasion ormetastasis of TCC. These suggested that the expression of MMP-2,9 were a predictorfor poor prognosis of TCC.