| The large colon cancer is one of the most common malignant tumors inthe world. Its outbreak rate reside thirdly only next to lung cancer andthe mammary glands cancer. The cause of the disease isn't clear andprognosis is worse. Five years livability is not more than 40%. In ourcountry, with the elevation of life level and the change of meal structure,the outbreak rate is up-trend year by year. In the last few years, thefunction of telomerase in cell ageing and canceration is valued by peopleincreasingly. Modern researcher regard commonly:The activation oftelomerase is the key step in the tumor formation process.This article takes Liandai recipe as the subjected to study theinfluence of Chinese herbal medicine compound prescribe to telomeraseand the expression of related gene.TO find a new path of the Chinese herbalmedicine suppression the cancer from the angle of the molecular biology,which has an important meaning to the mechanism of Chinese herbalmedicine suppression further cancer. This can establish a certainfoundation in order to study Chinese herbal medicine suppression to cancerfrom a deeper layer and provide a basis for the development new medicine.1,The clinical trial studyPurpose:To Observe the clinical curative effect of Liandai recipe to thelarge colon cancer who had chemotherapy after not radical surgery and theinfluence of Liandai recipe to telomerase and the expression of relatedgene hTERTmRNA.Research objectMethod:(1) 35 patients were come from the First affiliated hospital ofthe GuangZhou university of Traditional Chinese Medicine from August of2005 to August of 2006. They were hospital patients after a surgicaloperation or were outpatients.Study method:(1) To observe telomerase activity and the expression of hTERTmRNA intumor local organization of 35 large colon cancer. In the meantime, tocompare the difference of telomerase activity and the expression ofhTERTmRNA between cancer side organization and the normal organization.(2) Treating group of 20 patients take Liandai recipe decoction 50mls(which contained coptis chinensis 6g, Fructus Evodiaelg, IndigoNaturalis lg) twice a day extraly without changing Supportive treatment. Comparing group had the same Supportive treatment without takingLiandai recipe decoction. The course of treatment is one month, the twogroup had not take other drugs which had siminar function as Liandai recipedecoction.Result:(1)Confirming the higher telomerase activity and the expression of hTERTmRNAin tumor local organization of large colon cancer. The telomerase activityand the expression of hTERTmRNA are up-trend among normal colonorganization,side tumour organization,Adenomatous polypi and the largecolon cancer organization. They have no relativity with age,sex,diseasedregion in the large colon cancer and pathologic type, but they have aup-trend with anatomy grading and clinical stage.(2)After treatment, symptoms and signs of treating group were easedcomparing with control group. The telomerase activity and the expressionof hTERTmRNA of treating group in tumor local organization of large coloncancer were lower than control group.Conclusion:Liandai recipe can ease the symptoms and signs of colon cancer patients.One of the possible mechanisms is to change telomerase activity and theexpression of hTERTmRNA which have close relativity with tumors.2,The animal research studyPurpose: To observe the influence of Liandai recipe to the telomeraseactivity and the expression of hTERTmRNA in local tumour organizationof experiental naked mouse.Method: To replicate the model of colon cancer of naked mouse which wereinjected HT29 colon cancer cell subcutaneously. Each naked mouse waslavaged after the first injection for one month. Chinese herbs treatinggroup was lavage with Liandai recipe(2g/kg); western drugs treating groupwas lavage with AZT(150mg/kg), blank control group was lavaged with 0.09%Sodium Chloride. To dynamicly observe the change of tumor volume of nakedmouse. To detect telomerase activity with TRAP—ELISA and the expressionof hTERTmRNA with fluorescent quantitation PCR. TO analysis the resultswith statistics method.Result:(1)There was no significant difference between the three groups abouttumor volume of naked mouse subcutaneously after seven days after thefirst innoculation.They were for next expreriment.(2)There was no significant difference between treating group abouttumor volume of naked mouse subcutaneously after one month;There isdifference between treating group and control group about tumorvolume of naked mouse subcutaneously.(3)There was no significant difference between treating groups about thechange of telomerase activity and the expression of hTERTmRNA;but there was significant difference between treating groups andcontrol group about the change of telomerase activity and theexpression of hTERTmRNAConclusion: Liandai recipe can restrain the increase of transplantationtumor of naked mouse of HT29 cell strain of colon tumour. One of thepossible mechanisms is to reduce thethe expression of hTERTmRNA and lower the telomerase activity in localtumor organization.
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