Study On The Expression Of CCCTC-binding Factor (CTCF) And Telomerase HTERT In Human Endometrial Cancer Tissues

PART ONE RESEARCH ON DETECTION OF CTCFANDTELOMERASE HTERT PROTEIN IN HUMANENDOMETRIAL CANCER TISSUES BY WESTERNBLOTTING TECHNIQUEObjective: To test and contrast the expression of CTCF andtelomerase gene hTERT protein between endometrial carcinomas andnormal endometrial tissues; To respectively analysize the associationbetween expression of CTCF or telomerase hTERT protein and clinicalpathological parameters in human endometrial cancer tissues;To describethe relationship beween CTCF and hTERT in endometrial carcinomas; Inorder to lay the foundation for clinic.Methods: The control group(30healthy women normal endometrialsamples including15proliferative endometrium and15secretoryendometrium)and experimental group (45patients with endometrial cancer) were randomly selected which their expression levels of CTCF andtelomerase hTERT protein among two groups were determined by Westernblot.Results: CTCF and hTERT protein were both expressed in twogroups.The positive expression percentage both CTCF and hTERT inexperimental group was higher than the control group.The relativeexpression levels of two proteins were both exsited statistical differencesbetween experimental group and control group.The transcript of CTCF wasassocicated with endometrial cancer pathological stages and clinicalstages,while not with histological subtypes.In contrast,the expression ofhTERT has nothing to do with pathological stages, histological subtypesand clinical stages.In endometrial cancer,the expression of CTCF waspositively correlated with hTERT.Conclusion: CTCF and telomerase hTERT are differently expressedbetween endometrial carcinoma and normal endometrial tissue,alsodifferent in endometrial cancer clinical parameters under two differentexpression.Both joint detection in endometrial cancer has certainsignificance to endometrial carcinoma. PART TWO RESEARCH ON DETECTION OF CTCFANDTELOMERASE HTERT MRNA IN HUMAN ENDOMETRIALCANCER TISSUES BY RT-PCR TECHNIQUEObjective: To detect and contrast the mRNA expression of CTCF andtelomerase HTERT between endometrial carcinomas and normalendometrial tissues.Methods: From30cases of human normal endometrium specimens(15cases of proliferative endometrium,15cases of secretory phaseendometrium) separately selected the WB experimental CTCF positivesamples and hTERT positive samples to separately detect CTCFmRNA andhTERTmRNA expression; from cancer tissue samples from45cases ofendometrial carcinoma in patients separately selected the WB experimentalCTCF positive samples and hTERT positive samples to separately detectCTCFmRNA and hTERTmRNA expression; separately extracting RNAand using semi-quantitative RT-PCR to detect the mRNA expression.Results: The mRNA relative amout of CTCF and hTERT was exsitedstatistical differences between control group and experimental group.Conclusion: There exsits differences between experimental group andcontrol group on the mRNA expression of CTCF and hTERT.Using of thisdifference can be providing a certain basis for clinical diagnosis andtreatment.