| ObjectiveSystemic lupus erythematosus is a severly threatening human health disease in which the therapy of traditional Chinese medicine had characteristic and good therapeutic effect. In order to deeply understand the TCM effective emechanism to SLE, look for the more TCM and western medicine integrated therapeutic methods to SLE We treated SLE with TCM herp capsule - the nourishing yin clearing heat capsule, observed the genomics and proteomics effects of the nourishing yin clearing heat Capsule in SLE and researched the gene and protein expression level -the microscopic substantial phannacologic effect foundation of the nourishing yin clearing heat capsuleMethods1. Preparation medicated serum of the nourishing yin clearing heat capsule and establish In vitro culture experimental system of PBMC: (1) To preparatehigh and equivalent dosage intragastric liquid of the nourishing yin clearing heat capsule according to the adult dose, Use these intragastric liquid to gavage white rat for 7 days, extracte blood from heart after 1 hr of the last time gavage, filtrating blood through micro-aperture filter membrane for later experimentaluse; (2) To observate the different nourishing yin clearing heat capsule' s medicated serum influence on anti-dsDNA body of PBMC of SLE, determination the best medicated serum effective conceatration, and take these influence on anti-dsDNA body of PBMC of SLE for a reference to judge effect of nourishing yin clearing heat capsule' s medicated serum,2. To observate the influence of the nourishing yin clearing heat capsule medicated serum on SLE PBMC in gene expression: extracte blood from internal heat due to Yin deficiency type SLE, to separate PBMC from venous blood, and then (1) To extract mRNA from PBMC after cell culture with the nourishing yin clearing heat capsule medicated serum (2) To reverse transcript mRNA into cDNA and amplificate, put the cDNA in genechips to detect, finally collect and anlysis the data3. To observate the anti-dsDNAbody of PBMC of SLE, determination the best medicated serum effective concentration of the nourishing yin clearing heat capsule medicated serum on SLE PBMC in protein expression: extracte blood from internal heat due to Yin deficiency type SLE, to separate PBMC from venous blood, and then (1) To extract protein from PBMC after cell culture with the nourishing yin clearing heat capsule medicated serum (2) put the proteinin proteinchips to carry out SELDI-TOF-MS detection, finally collect and anlysis the data4. set up three control groups (decamethasone, control serum and blank control group) go along the same experimental procedureResults1. The nourishing yin clearing heat capsule medicated serum and decamethasone could effectively reduce the anti-dsDNA body concentration of PBMC of SLE,2. The influence of the nourishing yin clearing heat capsule medicated serum,decamethasone and control serum in gene expression to PBMC of SLE(1) In gene expression the nourishing yin clearing heat capsule medicated serum had definely regulated affection to PBMC of SLE up- regulated 51 genes, down- regulated 92 genes; Decamethasone up- regulated 85 genes, down-regulated 12 genes, control serum up- regulatedl 47 genes, down- regulated 100 genes(2). The pairwise comparison of influence the medicated serum, decamethasone and control serum to gene exgression of PBMC of SLE:The medicated serum and decamethasone up- regulated identically 2 genes; down- regulated identically 12 genes; neither up- regulated nor down- regulated identically 112 genes, medicated serum down- regulated but decamethasone neither up- regulated nor down- regulated 12 genes, medicated serum up-regulated but decamethasone neither up- regulated nor down- regulated 9 genes, decamethasone up- regulated but medicated serum neither up- regulated nor down- regulated 14 genes, decamethasone down- regulated but medicated serum neither up- regulated nor down- regulated decamethasone, in contrary regulated between medicated serum and decamethasone o gene.The medicated serum and control serum up- regulated identically8 genes; down- regulated identically 16 genes; neither up- regulated nor down-regulated identically 205 genes, medicated serum down- regulated but control serum neither up- regulated nor down- regulated 3 genes, medicated serum up- regulated but control serum neither up- regulated nor down- regulated 8 genes, control, serum up- regulated but medicated serum neither up- regulated nor down-regulated 33 genes, control serum down- regulated but medicated serum neither up- regulated nor down- regulated decamethasone 7 genes, incontrary regulated between medicated serum and decamethasone o gene.The decamethasone and control serum up- regulated identically 6 genes; down- regulated identically 25 genes; neither up- regulated nor down-regulated identically 205 genes, decamethasone down- regulated but control serum neither up- regulated nor down- regulated identically 8 genes, decamethasone up- regulated but control serum neither up- regulated nor down-regulated 27 genes, control serum up- regulated but decamethasone neither up- regulatednor down- regulated 3 genes, control serum down- regulated but decamethasone neither up- regulated nor down- regulated 7 genes, in contrary regulated between control serum and decamethasone o gene.(3) Some genes slected from gene exgression: 6 genes which medicated serum, decamethasone and control serum down-regulated identically are IRF1,FGFR4,POLA2,LOC51042,TGM1å’ŒCEPT1; 1 gene which decamethasone and control serum down-regulated identically medicated serum neither up- regulated nor down- regulated isPLAGL2, 1 gene which decamethasone and control serum up-regulated identically medicated serum neither up- regulated nor down-regulated is SMhRCA2, 2 gene which medicated serum and decamethasone up-regulated identically control serum neither up- regulated nor down-regulated are SC65 and BPNT1, 1 gene which control serumup-regulated medicated serum and decamethasone neither up- regulated nor down- regulated identically is ZNF75, 7 gene which decamethasone up-regulated medicated serum and control serum neither up- regulated nor down- regulated identically are SAS, CD163,NPTXR,HOXB3,RPLPO,ILIR1 and 1 unnamed gene, 1 gene which decamethasone down-regulated medicated serum and control serumneither up- regulated nor down- regulated identically is LOC51765, 1 gene which medicated serum up-regulated decamethasone and control serum neither up- regulated nor down- regulated identically is FGFR1, 1 gene which medicated serum down-regulated decamethasone and control serum neither up- regulated nor down- regulated identically is DDX6.3. The influence of the nourishing yin clearing heat capsule medicated serum,decamethasone and control serum in protein expression to PBMC of SLE(1) The influence of the nourishing yin clearing heat capsule medicated serum-in protein expression to PBMC of SLE: comparative to blank control 3 protein (7027, 4216 and 4236Da)were consisded to have statistical difference, analysed by professional software, 33 protein(7027, 8590 and 7675Da) could be composed a discrimination model that could distinguish the difference between medicated serum and blank control.(2) The influence of the decamethasone in protein expression to PBMC of SLE: comparative to blank control 1 protein (312Da)were consisded to have statistical difference, analysed by professional software, 4 protein(3864,2929,3651,3676Da) could be composed a discrimination model that could distinguish the difference between decamethasone and blank control.(3) The influence of the control serum in protein expression to PBMC of SLE: comparative to blank control 11 protein (7027,2798,4236,5270,4469,2908,4436,3104,1280,2311,4085Da)were consisded to have statistical difference, analysed by professional software, 2 protein(7027 and 5270Da) could be composed a discrimination model that could distinguish the difference betweencontrol serum and blank control(4) The influence comparated between nourishing yin clearing heat capsule medicated serum and control serum in protein expression to PBMC of SLE: 1 protein (2800Da)were consisded to have statistical difference, analysed by professional software, 3 protein(2800, 355land 5877Da) could be composed a discrimination model that could distinguish the difference between medicated serum and control serumConclusion1. The nourishing yin clearing heat capsule has his own phannacologic effect to internal heat due to Yin deficiency type SLE, parts of these effect of the nourishing yin clearing heat capsule that were revealed by gene expression especially are simillar to effect ofdecamethasone to SLE; 2. The yin clearing heat capsule exert their own effect through regulating a lot of genes and proteins these effect that act as wholism of TCM has complex impact to; 3. We could have some new chances to research mechanism prevention and control of SLE through studing the effect of medicated serum, decamethasone and control serum on some genes and proteins of internal heat due to Yin deficiency type SLR.
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