The Preliminary Research On Functions Of HPWTSR And Its Relationship With The Expression In Colon Cancer

Objective: To study the functions of hPWTSR and its expression patterns in colon carcinoma (CC), evaluate the potentiality of hPWTSR as a marker in the diagnosis and therapy of CC. Methods: The subcellular localization of hPWTSR was investigated in HEK293 cell line by fluorescence micrography. The effects of hPWTSR overexpression on CC HT-29 and LoVo cell lines were studied using pcDNA-hPWTSR recombinant expression plasmid. Cell cycle and apoptosis were detected by FCM. The influences of hPWTSR on cell adhesion and invasion ability were determined by methods of Matrigel agents and Transwell system. The correlationship between hPWTSR expression and carcinoma occurrence was revealed by analysis of the expression patterns of hPWTSR in CC cell lines and pathology tissues. Results: The EGFP-hPWTSR fusion proteins were located to cell membrane as particles. Overexpression of hPWTSR in both CC cell lines didn't result in evaluable changes of cell cycle. Overexpression of hPWTSR induced apoptosis of CC LoVo cell lines with high malignity although there was no similar effect presented in CC HT-29 cell lines with low malignity. Overexpression of hPWTSR promoted the adhesion ability, and restrained the invasion ability of two CC cell lines. The RT-PCR assay of expression pattern revealed that the expression of hPWTSR in CC pathology tissues was lower than that in corresponding normal tissues, which was further lower in patients with metastasis foci. And the expression of hPWTSR in CC LoVo cell lines of high metastatic potentiality was obviously lower than that in CC HT-29 cell lines of low metastatic potentiality. However, there was no mutation identified by the following sequencing. Conclusion: The subcellular location of hPWTSR suggests that it might be a cell surface protein. Overexpression of hPWTSR presented no significant effect on cell cycle of two CC cell lines suggest it might not be a proliferation related gene. Overexpression of hPWTSR induced apoptosis of CC LoVo cell lines with high malignity suggests that hPWTSR might affect growth of malignant tumor. Overexpression of hPWTSR improved the adhesion character and prevented the invasion character of tumor cells suggest that hPWTSR might relate to tumor metastasis. Moreover, pathology tissues and colon carcinoma derived LoVo cell lines of high metastatic potentiality presented a lower expression pattern of hPWTSR. These data suggest that hPWTSR might play a role in colon carcinoma occurrence, progression and metastasis. So the expression pattern of hPWTSR might be a potential colon carcinoma marker of diagnosis and therapy, and might be related to the metastatic potentiality and prognosis of colon carcinoma. Further study is necessary to clarify them.