Protective Effects Of Portulaca Oleracea L. Flavonoids Components On Nerve Tissues And Its Mechnism

Aim: The anti-hypoxia effects of the Portulaca Oleracea L. are well defined, however the effective components in the flavonoids remain elusive. The present study investigated whether the flavonoids components which we extracted from the PO total flavonoids have the neuroprotective effects and its molecular mechanism. Methods: After hypoxic mouse and hypoxic endothelial cells were administrated with aqueous solution of components of portulaca oieracea L. flavonoids, the survival time of the mice, the cell viability, the mouse cortex histopathological damages were detected. At the same time the expression of HIF-laand its downstream genes were determined by methods of MTT,RT-PCR,Western blot,ELISA,immunohistochemistry and transfection of antisense nucleotide. Results: 1) The components of the P prolonged the survival time of the hypoxia group, relieved the brain damage and increased the expression of the HIF-1 a, EPO and its mRNA in the cortex of the hypoxia group;2)The components of the P elevated the hypoxic cell viability, decreased the level of the LDH of the cell culture medium and relieved the cell apoptosis.3) Conclusion: We believe that components of P total flavonoids constitute a common mechanism that is involved in the hypoxic neuroprotective effects. Also, we are more convinced that this regulation of the HIF-1-EPO expression can be the result of the P extracts exhibition of its protective effects.