Studies On The Effects Of Total Flavonoids From Portulaca Oleracea L.on Hypoxia Tolerance In Mice

Objective: To find out the effective component of portulaca oleracea L. on the mice in anoxia and mechanisms of anti-hypoxia effect of the herb. Methods: We adopted mice after administration in the closed bottles to observe survival time. Or the mice subjected to closed chamber blew with the mixture of 90% nitrogen and 10% oxygen. Then the mRNA expression and the content of EPO and HIF-1α in tissues were assayed, the RBC,HGB and HCT were estimated, the LD and LDH were assayed, the activity of PFK , PK and ATP content were determinated. Results: the total flavonoids obviously enhanced the survival time of anoxia mice, elevated the expression of EPO mRNA, increased the content of HIF-la and EPO, ATP content and the activities of PFK, PK,LDH and significantly elevated RBC, HGB and HCT. Conclusion: the total flavonoids of portulaca oleracea L. have the best anti-hypoxia effect. The mechanism might be elevated the HIF-la in histocyte through which accelerate the expression of EPO level, enhance the body's ability of carrying and transporting oxygen and increase the activity of key enzymes in glycolysis in hypoxia mice, and elevated the ATP level to remission the energy metabolism in hypoxia mice.