| Quality of medical materials is one of the most decisive factors to the quality and potency of drugs, and quality control to be the core solution to safeguarding the security, effectiveness, stability and controllability of traditional Chinese medicines(TCM)and realizing the modernization, industrialization and internationalization of the industry, also the procedure with most challenge to resolve in the production. The past decades witnessed not only series of promising research on quality control conducted to form fine foundations for Chinese herbs security and quality control, but also disadvantages and shortcoming existed in the present research. Due to the fact that curative effects of the TCM are comprehensive interactions between different materials, the accepted quality assessment system by judging the content of active ingredient of the medical materials proved inadequate on objectivity and hard to be accepted by theories on TCM. Thus, the basic step is to set up a normalized quality assessment criterion to fit the principles in TCM and the acceptance of modern medicine. Quality of the medical materials from diversified regions is determined by its species, place, climate, soil, harvest period, drug use part and processing methods. Astragalus membranaceus and Codonopsis pilosula, as infinitive crude materials in China, show a mixed species and quality diversity for historical and geographical reasons. Quality assessment of materials in A. membranaceus and C. pilosula is the heated topic for research to select fine variety, widen and promote rational exploitation and use of medical resources, and guarantee secured effectiveness and controlled stability of drugs. The paper guided by the quality assessment index on A. membranaceus and C. pilosula in Chinese Pharmcopoeia (2005, 2010), set up a comprehensive assessment system integrating multiple indexes, then applied the system into the quality assessment to A. membranaceus and C. pilosula with diversified origins, analyzed the main factors affecting their quality, safeguarding the security, effectiveness, stability and controllability of the quality. Besides, owning to the clinical use of A. membranaceus and C. pilosula on therapy to chronic intractable disease and the raw antioxidant and antimicrobial extract of them, the paper also implemented study on the antioxidant and antimicrobial activity of their ethanol extracts, and concluded the referential results to the exploration of curative and healthcare mechanism of A. membranaceus and C. pilosula and the assessment and use of the resources. Major research findings concluded include:1,set up the fingerprint of HPLC-ELSD for A. membranaceus, there are 15 common peaks in the fingerptint; tested the total ash, acid insoluble ash, watery extract and contents of astragaloside IV referring to the assessing indexes of A. membranaceus in Chinese Pharmcopoeia (Edition 2005, 2010), set up the quality assessment system of A. membranaceus; applied the system into the quality assessment of A. membranaceus from various origins and concluded that quality of A. membranaceus from different habitats was different. Multi-indices to assess the quality of A. membranaceus could make assessment result more accurate.2,contents of astragaloside IV in A. membranaceus samples from different places and the HPLC chromatogram proved certain diversities; illustrated on the effects of annual mean temperature, minimum temperature, maximum temperature, annual accumulative temperature, annual precipitation, annual sunshine duration and frost-free period to contents of astragaloside IV in different places; SAS stepwise regression over the above elements demonstrated that annual maximum temperature, annual sunshine duration and average accumulative temperature evidently affected contents of astragaloside IV and high maximum temperature and average temperature proved favorable to the content accumulation.3,Both A. membranaceus and C. pilosula samples have 17 types of amino acid and trace element, among them 7 are fertile and complete amino acid essential for human. Samples acid ration approximated the number of reference protein model proposed byWHO/FAO (E/E+N40%, E/N0.6), which proved that A. membranaceus and C. pilosula are important resources of amino acid and the amino acid total content and necessary content varies from places. Samples from different places contain various trace elements of V, Fe, Co, Ni, Cu, Zn, Se and Mo. Research on amino acid and trace elements of their samples helped to construct the relations between their pharmacology and health care capacity, as well furthered the quality assessment to A. membranaceus and C. pilosula.4,set up the fingerprint of HPLC-ELSD for C. pilosula; tested the total ash, acid insoluble ash, lobetyolin and atractylenolide III referring to the assessing indexes of C. pilosula in Chinese Pharmcopoeia, set up the quality assessment system of C.pilosula; applied the system into the quality assessment of C. pilosula from various origins and concluded that quality of C.pilosula from different habitats was different. Multi-indices to assess the quality of C. pilosula could make assessment result more accurate.5,research on DPPH radical scavenging activity of A. membranaceus and C. pilosula ethanol extract from different places concluded the difference in their activity. A dose-response relationship existed between the concentration of their ethanol extract and DPPH free radical scavenging capacity, and the former surpassed the latter in the capacity.6,Research on A. membranaceus and C. pilosula ethanol extracts antimicrobial activity concluded that A. membranaceus extract attained inhibition to Staphylococcus aureus, Staphylococcus epidermdis, Branhamella catarrhalis, Streptococcus hemolysis-α, Streptococcus hemolysis-β, Bacillus anthracis, Klebsiella pneumoniae, Escherichia coli and Bacillus subtilis, more evident to Streptococcus hemolysis-β, Bacillus anthracis, Staphylococcus aureus and Bacillus subtilis., and ethanol extract of C. pilosula attained inhibition to Salmonella typhi and Proteus sp. besides the nine fungi mentioned above.
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