| Radix astragali is the roots of Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao,or Astragalus membranaceus(Fisch.)Bge.Besides organi c acid,microelement and sterols,Huangqi mainly consists of flavonoid,saponin s and polysaccharides.In the clinical,Huangqi is usually used for treating card iovascular and digestive diseases.Objective Daxinganling is a new region for Radix Astragali,at present has n ot been accepted by the public.The researches of Daxinganling Huangqi report er rarely.Establishing analytical method of flavonoid,saponins and polysacchari des.These methods can be used for quality study of Daxinganling Huangqi.O n the basis of quality of Daxinganling Huangqi,we can develop composite ext racts.The research could promote and drive the industrialization development o f Huangqi and the economy development in DaxinganlingMethods(1)Taking the extraction conditions of flavonoid,saponins and polysac charides investigate to determine the assaying in roots,processed products,ste ms and flowers of Radix Astragalis.(2)Using HPLC to establish the methods of assaying and feature finger-print of flavone chemical constituents,determine flavonoids component in Astragalus membranaceus and its processed products f rom different sources.Comparing the specific chromatogram of flavone of Hua ngqi and its processed products in different sources.(3)Determining the content of moisture,ash and water extract according to the method in Chinese Pharm acopeia.(4)Combined with the analytical methods determinating Astragalus me mbranaceus processed products.(5)Taking the receiving rate and the content of flavonoid and saponins as evalution index,receiving the best extraction and p urification technology of extract.Preparing three extracts and formulating the q uality standard of Huangqi extractResults(1)This study have established the determination method of polysacchar ides by phenol-vitriol.(2)This study found that the content of total polysacchar ide in Radix Astragali is Daxinganling>Shanxi>Gansu>Neimenggu,the content of total polysaccharide in Astragalus membranaceus processed products is Radi x Astragali>Fry Astragalus>Honey Astragalus,the content of total polysaccharid es in different part of Radix Astragali is the root>the flower>the stem.Measuri ng the total flavonoids in Radix Astragali and its processed products by UV s pectrophotometry,we found that the content of total flavonoids in Radix Astrag ali from different areas is no significant difference,the content of total flavono id in Astragalus membranaceus processed products is Fry Astragalus>Radix Ast ragali>Honey Astragalus,the content of total flavonoid in different part of Rad ix Astragali is the flower>the stem>the root Methods with 5%vanillin-acetic a cid and perchloric as the deloper,colorimetric method was applied to assaying the total saponins in Radix Astragali and its processed products.This study fo und that the content of total saponins in Radix Astragali is Neimenggu>Shanx i>Gansu>Daxinganling,the content of total saponins in Astragalus membranace us processed products is Radix Astragali>Fry Astragali>Honey Astragalus,and the content of total saponins in different part of Radix Astragali is the stem>th e flower>the root.(2)Measuring the content of flavonoids constitutes by HPLC,the content of calycosin-7-glucoside in Radix Astragali is Daxinganling>Neime nggu>Shanxi>Gansu,the content of calycosin in Radix Astragali is Daxinganlin g>Shanxi>Gansu>Neimenggu,the content of formononetin in Radix Astragali is Gansu>Shanxi>Daxinganling>Neimenggu,while the content of calycosin-7-gluc oside is the highest.Based on the HPLC mehod of flavonoid constitute to assa y Astragalus membranaceus processed products found the content of calycosin-7-glucoside,calycosin and formononetin of Radix Astragali is highest.The degre e of similarity comparison feature chromatography between Daxinganling Huang qi and Shaxi Huangqi is close.Analyzing the feature chromatography of Astra galus membranaceus processed products found that Heber Huangqi was close to Dongbei Huangqi,the degree of similarity Huma Huangqi is low.(3)The assa ying of moisture in Radix Astragali was 6,57%?8.11%;the assaying of ash wa s 3.06%?4.15%;the assaying of extractum was 21.71%-47.27%.(4)The influenc es of processing and storaging ways in Astragalus membranaceus were not the same.Honey Huangqi has lager decline in the quality than Fry Huangqi.The storage ways has stable changes.(5)The best extraction condition of Astragalu s membranaceus compound extract as followed:medicinal materials was extract ed with 70%ethanol for 2 times(2 hours every time),then the extracting solut ion is merged for rotatory evaporation till no alcohol taste.The solution was tr ansited by D101 macroporous resin column,and cleared of water(4B)and 70%ethanol(4BV).The moisture of three extract is no more than 5.0%,the residue of three extract is no more than 0.8%.The assaying of total flavonoid and to tal saponins was aboved 50.0%.Conclusion(1)The established determination method of total polysaccharide,tot al flavonoid and total saponins can be used for comparing the differences in th e root of Huangqi,the processed products,the stem and flower.(2)Variance in Radix Astragali from different sources can be proved the geographical advanta ge of Daxinganling.(3Based on Ch.P adding total polysaccharide,total flavonoi d,total saponins and the feature-print chromatography,This study can be appli ed to comparing the quality of Radix Astragali.(4)The content of total flavon oid and total saponins was aboved 50.0%.And the established quality standard of extract can be used for keeping the moisture and extractum in a region. |
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