Effects Of Reinforcing Kidney On The Expression Of GDNF,GFR_α-1in Granulosa Cell Of Superovulation Cycle

Objective:To investigate the effects of reinforcing kidney on the expression ofGDNF,GFRα-l in granulosa cell of superovulation cycle, In view of GVBD,extrusion ofPB1and oocyte maturation paracrine, to explore the mechanism of reinforcing KidneyChinese herbs Erzhitiangui(ETG) Granule to improve the treatment outcome of insuperovulation cycle.Method: Clinical Study:80infertile women with tubal factor undergoing vitrofertilization-embryo transfer (IVF-ET) were randomized into the study group, treated withETG and Gn(40cases), and the control group, treated with placebos granules and Gn (40cases). The long term protocol was adopted. The E2and P secretion in granule cell aftercultured for24h,48h in vitro was observed to learn about the secreting function of granulecell. The expression of GDNF,GFR-1mRNA in luteinizing granulosa cells were alsodetected. The syndrome scores of TCM, administration time and number of Gn, number ofretrieved oocytes, fertilization rate, high quality embryo rate and pregnancy rate wereevaluated.Experimental Study:165Kunming female mice of8-9weeks old were allocated intothree groups randomly: control group(group HMG＋HCG＋NS), HMG and HCG weregiven for ovarian stimulation, performed abdomen injection with0.9%salt solution ofsame as study group on the same time schedule; study group(group HMG＋HCG＋ETG);ETG combined with HMG and HCG were given; blank group(group nothing). Three groupwere divided into1,2groups each.1group were taked denuded on germinal vesiclestage,observed the germinal veicles breakdowm and extrusion of the first polar body, The expression of GDNF,GFR-1mRNA in the oocytes were also detected;2group were retrieved to evaluate the ability of merogenesis and forming the embryo.Result:Symptoms of kidney asthenia of the study group was significantly improved.The level of E2per occtye, the ratio of high quality occtye and embryo, the clinicalpregnancy rate were higher than those of the control group. The difference werestatistically significant (P＜0.05). The E2and P secretion in granule cell after cultured for24h,48h in vitro were higher than the control group. The expression of GDNF,GFRα-lmRNA in granule cell of the study group was higher than that of the control group, Thedifference were statistically significant (P＜0.05). The study group was significant higherthan control group in the frequency of the germinal veicles breakdowm and extrusion ofthe first polar body. The expression of GFRa-l mRNA in in the oocytes of the study groupwas higher than that of the control group. The number ability of merogenesis and formingthe embryo of fertilized ovum in control group were lower than those of the study andblank group. The difference was statistically significant (P＜0.05).Conclusion:1. Reinforcing Kidney Chinese herbs combined with western medicinecan decrease the dosage of Gn obviously and improve symptoms of kidney asthenia, thequality of oocyte, the ratio of high quality occtye and embryo, the ratio of pregnancy.2.Reinforcing Kidney Chinese herbs can also improve the secreting function of granule cell,the expression of GDNF,GFRα-1mRNA in luteinizing granulosa cells. That maybe oneof the mechanism of reinforcing Kidney Chinese herbs to improve the ratio of pregnancy.and raise the quality of oocytes.3. Reinforcing Kidney Chinese herbs can improve thegerminal veicles breakdowm and extrusion of the first polar body, the ability ofmerogenesis and forming the embryo of fertilized ovum, and the expression of GFRα-1mRNA in oocytes., which may suggest that Reinforcing Kidney Chinese herbs raise thequality of oocytes,embryo and the ratio of pregnancy by improve the germinal veiclesbreakdowm, extrusion of the first polar body, the expression of GDNF,GFRα-1mRNAin luteinizing granulosa cells and the expression of GFRα-1mRNA in oocytes. Thatmaybe another one of the mechanism of reinforcing Kidney Chinese herbs to improve theratio of pregnancy.