Mechanism Of JAK₁-STAT₃ Signal Transduction In Electro-Acupuncture For Treating Facial Nerve Injured

Objectiveto discuss the molecular signal mechanism of electro-acupuncture for treating fac-ial nerve injured,we do the research start from JAK1-STAT3 signal pathway which can provide the scientific experimental evidence for improving the clinical curative ef-feet.MethodsExperimental animals provided by a special committee of Sichuan Province, rab-bits of Japan species, male and female each half, weight 1.9-2.lkg,80-85 days old. Sixty-six rabbits were divided eleven groups of which each group include six rabbits randomly. Except the preoperative control group, sham group, other groups squeezed the right side of the facial nerve with a special stop bleeding clamp. EA group were treated in the immediate postoperative day,5 days a course of treatment, continuous treatment of 3 courses. Preoperative control group derived after 3 days of feeding. Self-healing group model derived after five weeks. Observed the effects about the tis-sue morphology of the facial nerve injured model, and the expressive level of CNTFR,CNTFR mRNA,JAK1,STAT3 after EA "Dichang", "Jiache", "Yifeng", "Hegu" Points.Results1. The morphological quantitative:after 5 treatments, there were statistically signifi-cant differences between the model group and EA group in quantity and thickness of myelin sheath(P<0.01). after 10-15 treatments, the quantity and thickness of myelin sheath of EA group began to drop, there were no statistically significant differences between the two groups(P>0.05).2. The expressive level of CNTFR:There was the basic expression of CNTFR in preoperative control group, after 5 treatments, the expression of CNTFR decreased in EA group, there were statistically significant differences between the model group and EA group (P<0.01); after 10-15 treatments, the expression of CNTFR increased in EA group, there were no statistically significant differences between the two group-s(P>0.05).3. The expressive level of CNTFR mRNA:There was the basic expression of CNTFR mRNA in preoperative control group, after 5 treatments, CNTFR mRNA ex-pression decreased in EA group, there were statistically significant differences betwee-n the model group and EA group (P<0.01); after 10-15 treatments, CNTFR mRNA expression increased in EA group, there were no statistically significant differences be-tween the two groups(P>0.05).4. The expressive level of JAK1:after 5 treatments, JAK1 expression decreased in EA group, there were statistically significant differences between the model group and EA group (P<0.01); after 10-15 treatments, JAK1 expression increased in EA group, there were no statistically significant differences between the two groups(P> 0.05).5. The expressive level of STAT3:after 5 treatments, STAT3 expression decreased in EA group, there were statistically significant differences between the model group and EA group (P<0.01); after 10-15 treatments, STAT3 expression increased in EA group, there were no statistically significant differences between the two groups(P> 0.05).Conclusion1. myelin is negative related with the degree of facial nerve injured. The expressive level of CNTFR, CNTFR mRNA,JAK1,STAT3 is positive related with the degree of facial nerve injured.2. EA can significantly speed up the process of myelin sheath, and quickly regulate the expressive level of CNTFR,CNTFR mRNA,JAK1,STAT3 to normal levels, promote to repair nerves in acute stage.3. Excessive EA stimulation caused damage response, made myelination decrease and activate CNTFR, CNTFR mRNA, JAK1, STAT3 once again.4. EA can promote to repair facial nerves in acute stage. Excessive EA stimula-tions can cause damage response. It is necessary to grasp "the disease lasts" principle in EA treatment.