Effects Of Facial Nerve Damage Facial Nerve Nuclei Of Neuronal Apoptosis In Magnetic Resonance Spectroscopic Imaging Study

Objective To establish SD rats' two sides of facial nerve high level injured model,we cut the SD rats' two sides of facial nerve trunk on the high level, which Induced neurons apoptosis of two sides of facial nerve nuclear regiment, and provided experimental basis for detection neurons apoptosis inside the brain by magnetic resonance 1H-MRS.Methods Male SD rats of clean grade 20, make it cut off,which its two-side facial nerve trunk at stylomastoid foramen in the stretch of about 3mm, and the rats were evaluated for facial paralysis.Results On the injured side of 1 to 3week group there was complete facial nerve paralysis Both two sides of facial nucleus.Conclusion SD rat's two sides of facial nerve high level injured model may be induced to two sides of facial nerve complete paralysis, and it's very reproducible so it can provided experimental basis for detection neurons apoptosis inside the brain by magnetic resonance 1H-MRS.Objective To investigate the value of proton magnetic resonance spectroscopy (1H-MRS) technique for high level facial nerve injured in rats after two side of facial nucleus's apoptosis observed in vivo feasibility.Methods 24 SD rats were selected, and their were divided into normal control group and model group.1H-MRS data obtained from 24 SD rats in GE 3.0T MR scanner were retrospectively analyzed. By using Functool 4.5.5 quantification of N-acetyl aspartate (NAA), creatine (Cr), choline (Cho) were obtained.Results Model group, NAA, NAA/Cr, NAA/Cho were lower than the normal control group, there were statistically significant differences,P<0.05(Model group and normal control group NAA were 7840.0±2671.46,10173.18±1500.33, t=3.159, P=0.003; Model group and normal control group, NAA/Cr were 0.94±0.33,1.27±0.14,t=3.782, P=0.001; Model group and normal control group NAA/Cho were 1.46±0.39,1.76±0.45, t=2.076, P=0.046). Model group's Cho,Cho/Cr and normal control group were not statistically significant different, P>0.05(Model group and normal control group Cho was 5514.50±1801.51,6035.76±357.42,t=0.962, P= 0.343; Model group and normal control group Cho/Cr were 1.46±0.39,1.76±0.45, t=1.418,P=0.165).Conclusion The SD rat's two sides of facial nerve high level cut off neurons of facial nucleus can lead to the facial nucleus of apoptotic neurons, and' H-MRS technique can be used in vivo detection of the inner surface of the facial nucleus of apoptotic neurons.