Intervention Research Of The Bushenzhuanggu Capsule To The Mechanism Of Osteoarthris Of Experimental Knee Joint

Knee osteoarthritis(KOA) is the main cause of older knee pain and disability, its dysfunction due to discomfort as well as the quality of life of patients have adverse effects. According to TCM syndrome differentiation theory,the overall concept and the guidance of Chinese medicine treatment of the disease targeted significant effete,And long-term effeets,They could fundamentally control the disease's development a permanent cure.In this study, finishing in the system knowledge of medicine to the KOA on the basis of relevant literature, the establishmnent of animal models studies carried out to explore the Pathogenesis of the KOA and Chinese medicine treatment of KOA effectiveness and feasibility of Prevention and treatment.for Chinese medicine,KOA Provide reliable experimental data and solid theoretieal basis.Objective:To observe the efficacy of Bushenzhuanggu Capsule(BSZGC) for treatment of knee osteoarthritis(KOA),and explore the mechanism in the effect of BSZGC and the mechanism of KOA.Methods:In the experiment study,40 New Zealand rabbits were randomly divided into 4 groups:Group A(the normal group), Group B (the model group), Group C(the treated group) and Group D(the control group).KOA models were induceded in Group B, C and D by Hulth's technique. The rabbits in Group C were treated with BSZGC and Zhuangguguanjie pill (ZGGJP) was used in Group D.8 weeks later, to make CR of the knee. Observe the pathological changes of articular cartilage and by HE colouration under light microscope and scanning electron microscope, then mark it by Mankin. Immunohistochemistry was applied to observe the expression of MMP-1 and TIMP-1 and ADAMTS-4 in articular cartilages, Check chondrocyte apoptosis index(AI) by TUNEL.Measure expression rate of bcl-2 mRNA and bax mRNA by RT-PCR.The experimental data are processed with SPSS 16.0 statistical software, gross score compares the rank and examination. Comparison between groups with one-way ANOVA analysis of variance, compares the mean differences with SNK-q or LSD inspection.If P< 0.05, the differences are statistically significant. Rusult:Under light microscope and scanning electron microscope, Bushenzhuanggu Capsule could decrease macroscopic grading score, X-rayphotograph score, Mankin's score and relief structure of articular cartilage in OA knee by Hulth's technique,so it could prevent articular cartilage from OA degeneration. After treating with Bushenzhuanggu Capsule, chondrocyte apoptosis index(AI) were decrease,expression rate of bcl-2 mRNA were raised and that of bax mRNA were lowered. Conclusion:The Bushenzhuanggu Capsule can restrain chondrocyte apoptosis of knee Oteoarthritis,and relive damage of articular cartilage. The action may be caused by two approaches,which the first is decreasing grade of MMP-1,ADAMTS-4 and raising grade of TIMP-1, and the second is raising expression rate of bcl-2 mRNA and lowering that of bax mRNA. And adjust the ratio of Bcl-2 mRNA/Bax mRNA. This study of Chinese medicine for the prevention and treatment of knee osteoarthritis to provide a reliable experimenial data and solid theoretical basis.