Study On The Signal Transduction Of IκK/IκB/NF-κB In Deep Venous Thrombosis And The Interventional Mechanism Of Chinese Herbs

Objective:To investigate the pathogenesis of deep venous thrombosis(DVT) from the Signal transduction of Nuclear factor kappaB/Inhibitory kappaB/Inhibitory kappaB kinases (IκK/IκB/NF-κB), and interventional mechanism of Chinese Herbs on DVT, in order to provide new idea for prevention and cure of DVT.Methods:In the experiment, uses the vena cava inferior ligation law to establish the deep phlebothrombosis big mouse model.Wistar rats were divided into five groups which included Di Dang Tang group, Chuan Wang Xiao Yan tablets group, Fu Fang Dan Shen tablets group, model group, sham group. Then to observe the level of NF-KBP65,NF-κBP50,IκKα,IκBαkinases IκKα(I kappa B kinase a) and IκKβ(I kappa B kinaseβ) in vascular endothelial cell(VEC) and Vessel wall, through through immunohistochemical method and Quantitative PCR method, discussing their Variation,observe the pathological character under the light microscope, the electron microscope and transmission electron microscope.Results:Experiment shows that higher level of NF-κBP65,NF-κBP50,IκKα,IκKβin vascular endothelial cell(VEC) and Vessel wall of model group,lower level of IκBa in VEC and Vessel wall of model group, and more severe pathological changes in model group.Di Dang Tang can effectively reduce the expressions of NF-κBP65,NF-κBP50,IκKαand IκKβ, improve the expressions of IκBa and the pathological changes.Conclutions:The Signal transduction of IκK/IκB/NF-κB may participate in genesis and development of DVT, introduce the reciprocity of VECs and injury of vein. The Chinese herbs can intervene the Signal transduction of IκK/IκB/NF-κB to suppress inflammatory reaction and thrombosis.