Study On Protective Effects Of Huo Xue Tong Luo Broth On Vascular Endothelial Cells In Deep Venous Thrombosis

Objective: To research the mechanism of Huo Xue Tong Luo Broth in preventing deep venous thrombosis, mainly from the view of the protection of endothelial cells to study on interventional mechanism of Huo Xue Tong Luo Broth in deep venous thrombosis, in order to provide an objective basis for clinical treatment.Methods: Deep venous thrombosis model in rats was established through ligation of inferior vena cava and bilateral ligation of common iliac vein.The role of Huo Xue Tong Luo Broth was studied through the mouse ear model of acute inflammation and vascular endothelial cell injury model.Results: Huo Xue Tong Luo Broth can inhibit thrombus formation in rats (p<0.01), degree of inhibition of mouse ear edema (p<0.05), protect HUVEC cells which induced by glucose deprivation injury and oxidation injury.Conclusions: Vein ligation can effectively set up the model of deep vein thrombosis. Deep vein thrombosis and vascular endothelial cell injury has relevance. Huo Xue Tong Luo Broth can inhibit inflammatory cell infiltration, protect vascular endothelial cells, reduce thrombosis, removal of oxygen free radicals and reduce cell apoptosis. Huo Xue Tong Luo Broth can protect vascular endothelial cells which induced by glucose deprivation injury and oxidation injury. By protecting vascular endothelial cells , Chinese herbs could achieve the prevention and treatment of lower extremity deep venous thrombosis of the purpose.