Study On The CAMs In Deep Vein Thrombosis And The Interventional Mechanism Of Chinese Herbs

Objective:To investigate the pathogenesis of deep vein thrombosis(DVT) from the view of cell adhesion molecules(CAMs) and interventional mechanism of Chinese Herbs on DVT,in order to provide objective basis for clinical diagnosis and treatment.Methods:60 patients of DVT were divided into groups according to the syndrome differentiation type of traditional Chinese medicine(TCM) respectively.Based on the type of TCM,it was divided into the syndrome of pathogenic dampness-heat(A), the syndrome of blood stasis and dampness(B), 20 health people as control(C). All patients and healths were determined blood serum levels of ICAM-1, VCAM-1,PSGL-1,and discuss whether there is difference in the point of gender and traditional medical syndrome.In the experiment, 180 Wistar rats were divided into six groups which included Xiao Shuan Tong Mai group, Xue Fu Zhu Yu capsule group, Chuan Wang Xiao Yan tablets group, Haperin group,model group, sham group.Then to observe the pathological character, the serum level of ICAM-1 and the expression of the inflammation cells,VCAM-1,PSGL-1 in the vein walls.Results:The serum levels of ICAM-1,VCAM-1,PSGL-1 of patients were higher than those of the control(P<0.01).The serum levels of ICAM-1,VCAM-1,PSGL-1 in group A were higher than group B and C(P<0.01),there is no distinct difference in the point of gender(P>0.05).The level of blood serum of CAMs of mixed type is higher than that of center type and around type(P<0.05,P<0.01).The level of CAMs of center type is higher than around type,but there is no obviously difference(P>0.05).Experiment shows that pathological changes in models were more severe than sham, higher levels of VCAM-1,PSGL-1 and higher expressions of the inflammation cells in the vein wall(P<0.05,P<0.01).The level of blood serum CAMs is maximal on the first day after operation,and then declined gradually.Xiao Shuan Tong Mai can effectively improved the pathological changes, reduced the expressions of these CAMs and inflammatory cytokines(P<0.05,P<0.01 ).Conclutions:CAMs may participate in all pathological courses of DVT which involved in genesis,development,organization and recanalization. It can be as one of the objective index in the syndrome type of TCM.The mechanism of the treatment of DVT by Chinese herbs may be the intervention and modulation of the CAMs.