The Study On The Relativity Of Blood Viscosity And Ingredient Of Erythrocyte Membrane Of Coronary Heart Disease Angina Patients Who Were Qi Deficiency To Blood Stasis

Objectives:The coronary heart disease patients who were qi deficiency to blood stasis were observed in this article. The mechanism of the decreasement of red cell deformability and membrane fluidity of coronary heart disease patients were studied through the change of hemorheology and ingredient of erythrocyte membrane. The mechanism of blood stasis that happened in erythrocyte membrane was further looking for. And the results could be as the reference for diagnosis and treatment of coronary heart disease patients who were qi deficiency to blood stasis. The technology platform was established for studying of mechanism and target spot of action.Methods:The senile rats and young rats were observed in this article. Erythrocyte membrane was prepared. The change of blood viscosity and ingredient of erythrocyte membrane were determined. The research method for the relativity of them was established. Eighteen coronary heart disease patients who were qi deficiency to blood stasis, thirteen healthy old people and ten healthy young people were divided into coronary patient group and healthy old people control group and healthy young people control group. Many indexes such as biochemical indicator of plasma, whole blood viscosity, plasma viscosity, erythrocyte deformability and the content of cholesterol, sialic acid, hydrosulphonyl, MDA, activity of SOD, Na+-K+-ATP enzyme activity of erythrocyte membrane, constitute of membrane protein, change of form of erythrocyte membrane under laser scanning confocal fluorescence microscope were determined. The mechanism of causing blood stasis was investigated in this article.Results:(1) The whole blood viscosity and plasma viscosity of coronary patient are significantly higher than that of the healthy people (P<0.01,P<0.05); The red cell deformability index is lower than that of healthy people (P<0.01); The contents of cholesterol in erythrocyte membrane and plasma are significantly higher than that of the healthy people (P<0.01,P<0.05). The contents of sialic acid and hydrosulphonyl in erythrocyte membrane are significantly lower than that of the healthy people (P<0.01,P<0.01). SOD and Na+-K+-ATP enzyme activity of erythrocyte membrane are decreasing and the content of MDA is increasing. But there are not statistically significant. (2) Compare with the young people, there are some changes of red cell morphology and membrane skeleton in coronary heart disease patients who were qi deficiency to blood stasis. The content of F-actin is decreased and F-actin is depolymerization. The percentage composition of band 1 protein, band 3 protein, band 4.1 protein, band 4.2 protein, band 5 protein, band 6 protein and tropomodulin of coronary patients are significantly lower than that of the healthy young people. The percentage composition of band 1 protein, band 3 protein and band 4.2 protein of the healthy old people are significantly higher than that of coronary patients.Conclusions:The senile rats and young rats were as object of study. The research method for the relativity of blood viscosity and ingredient of erythrocyte membrane was established. The whole blood viscosity and plasma viscosity are increased significantly and the red cell deformability is decreased distinctly. The mechanism of decreasing antioxidant ability and membrane fluidity may has relationship with the following points:increasing the content of sialic acid and hydrosulphonyl, reducing the content of cholesterol in erythrocyte membrane, depolymerization of F-actin and the change of composition of membrane protein.