Study On Chemical Composition Of Thuja Occidentalis Cupressaceae And The Flos Of Pueraria Lobata (willd.)ohwi And The Flos Of Salvia Miltiorrhiza Bge.

Chemical composition of thuja occidentalis Cupressaceae, the flower of Pueraria lobata (Willd.) Ohwi and Salvia miltiorrhiza Bge. was studied.Chemical composition from chloroform fraction of thuja occidentalis cupressaceae was studied. Nine compounds were isolated by silica gel and reversed-phase coulmn chromatography. This structures were elucidated by means of spectroscopic techniques including 1D and 2D-NMR, 1HNMR,13CNMR,1H-1H COSY, HMQC, HMBC, DEPT HR-ESI-MS with chemical methods. Among nine isolated compounds, eight compounds were first separated from this species. The result were concluded as follows:Nine compounds were trans-commonuic acid, sucrose,β-sitostero glucoside, isocupressic fatty acid esters, isocupressic acid,deoxypodophyllotoxin, 15-oxolabda-8(17),13E-dien-19-oic acid and pimaradienoic acid respectively.The three samples of Ningde Fujian, Zhangjiajie Hunan, Yifengshi Jiangxi flower of Pueraria lobata were extracted with mixed solvent and analyzed with the GC-MS. Four kinds of compounds including weak polar, tarpane, sterane and polar compounds were all contained in Pueraria lobata origined from three areas. In all the samples, alkane was the main weak polar compounds (content of hentriacontane were the highest) and fatty acids was the main polar conpounds (content of hexadecanoic were the highest). Tetracycoloterpane was the main tarpane compound in the sample from Ningde Fujian and 17α(H)21β(H )-hopane was the main tarpane compound in the sample from Zhangjiajie Hunan and Yifengshi Jiangxi.ααα-20R-cholestane was the main sterane compound in the sample from NingdeFujian and Zhangjiajie andαββ-20R-24-Me-cholestane was the mainsterane compound in the sample from Yifeng Jiangxi.The samples of ?ower of Pueraria lobata and pueraria thomsonii Benth. from Yifengshi Jiangxi were also extracted with mixed solvent and detected by GC-MS. The results showed that both of them contained weak polar and polar compounds. However, neither terpane nor sterane was contained in ?ower of pueraria thomsonii Benth..The petroleum ether and chloroform extract of flower of Pueraria lobata from Yifengshi Jiangxi were detected by GC-MS. Both weak polar and polar compounds were detected in the two kinds of extracts. Both tarpane and sterane were detected in chloroform extracts, but they were not contained in petroleum ether extracts. Alkane was the main weak polar compounds. Content of hentriacontane was the highest in petroleum ether extracts and that of nonacosane was the highest in chloroform extracts. Fatty acids were the main polar compounds and content of hexadecanoic acid was the highest. There were more polar compounds in petroleum ether extracts than in chloroform extracts. 9.12 octadecdienoic acid and 9.12.15 octadecdienoic acid were healthful for human, and their contents were higher in petroleum extracts. Sterane, tarpane, ketones inpetroleum extracts were biological activities.Essential oil in ?ower of Pueraria lobata and pueraria thomsonii Benth. fromYifengshi Jiangxi was extracted by steam distillation and detected by GC-MS. The results showed that content of fatty acid mainly including hexadecanoic acid was the highest in essential oil.The solvents extracts of purple and white flower of Salvia miltiorrhiza from Shaanxi Tasly Plants Pharmaceutical (Shangluo, China) and Medicinal Botanical Garden northwest A&F university Shaanxi.were detected by GC-MS. As a result, 64 compounds were detected. Nonacosane was the main non-polar compound and hexadecanoic acid was the main polar compound.