Traditional Chinese Medicine XinWen Tong Yang Of Atherosclerosis Rat Il-6/STAT Signaling Pathways To Adjust Action Research

ObjectiveThrough the XinWen tong Yang theoretical analysis and experimental research, proposes the use of XinWen tong Yang method treatment AS. But at the same time, through XinWen tong Yang medicine and TCM, eliminating phlegm eliminating stasis Chinese medicine,Chinese traditional medicine,and dredgin g several common Chinese XinWen tong Yang the comparative study of Chinese XinWen tong Yang,clear of atherosclerosis rat IL-6/STAT3 signaling pathways leading to the function and mechanism, and attempts to reveal XinWen tong Yang herbal treatment AS the biological basis.MethodsHealthy purebred male SD rats,weighing 200-230 grams 120,3-4 months old,were randomly divided into normal control group,the blank model grou p,light blue extraction group,scallion white extraction group, Guizhi extraction group,ginger extract group, monkshood extraction group, Salvia extract group,Trichosanthes extraction group,to turtle worm extraction group, rhubarb extract group, probucol group, n=10.Prepared with reference to Guoyan Son g,etc.atherosclerosis model.Adaptive feeding week later,the normal control group fed basic diet and ordinary tap water, the model group and the treated group with high fat diet (4% cholesterol,0.5% sodium cholate,10% lard,0.2% propylthiouracil and94.3% of the diet) feeding.And at the beginning of the experiment in the right lower limb intram uscular injection of vitamin D3 (3×105u/kg body weight), repeated every 30 days after the injection.7 days except in the normal control group, The rest of the group underwent balloon injury of rat aorta surgery Group 3 days after modeling, starting dose,dose volume of 2ml/kg, the normal control group and blank model group were given saline.Onion extract group were 20m g.kg-1 administration,scallion white extract administered by 60mg.kg-1,Gui extract administered by 15mg.kg-1,ginger extract administered by 10m g.kg-1,Fuzi extract administered by 20mg.kg-1,Salvia extract by 10mg.kg-1 admin istration, Tricho -santhes extract administered by 10mg.kg-1, to extract turtle worms administered by 10mg.kg-1, rhubarb extract administered by 10mg.kg-1,probucol group administered by 10mg.kg-1;day 1,continuous admi-nistrati on for 16 weeks.16h fasting in the 16th week after the experiment, anesthe tized animals, abdominal aortic blood, the separation of the common carotid artery, in the initial segment of the aortic arch aortic specimens taken 0.5cm,10% paraformaldehyde solution, fixed for 12 hours; open the abdominal cavity, separatede -xtravascular tissue, cut along the midline dorsal aorta, physiol-ogical saline,-80℃refrigerator cold standby; indwelling two 5ml blood were separated serum stored in -80℃refrigerator cold standby.Through HE dyeing, oil red O dyeing analysis pathological changes and lipid deposition; Using real-time fluorescence quantitative PCR technology, Western Blot test TLR4, LXR in AS big lies the expression; aortic Immunohistochemical method to detect JNK/P-JNK, P38MAPK/P-p38 MAPK in AS big lies the expression, with ELISA aorta ERK1/2 method to detect the ERK1/2 P-with Western Blot test expression, P38MAPK/P-p38 MAPK in AS big lies the expression; aortic Using real-time fluorescence quantitative PCR technology detection AS big lies AGTR1mRNA expression; aortic vascular Adopt immunohistochemical method, the Western Blot test AS big liesκB P65 aortic NFκB/p-NF P65 expression; By ELISA, immunohistochemical method, real-time fluorescence quantitative PCR technology detection AS big lies aortic vascular IL-6 expre ssion; Western Blot test AS big lies JAK1/p-aorta JAK1, STAT3/p-STAT3 expression.conclusion1. Set extract, Xie white extract, cassia twig and extract, dry ginger extract, la teral extract, danshen extract, pericarpium trichosanthis extract, DeBie bug ext ract and rhubarb extracts all can affect IL-6/STAT3 in signal transduction rel ated the expression of cytokines.2. XinWen tong Yang Chinese medicine XinWen tong Yang between traditiona 1 Chinese medicine, physicians group compared with other drugs, has certain a dvantages, but only in part link, and has obvious advantages in overall showed no obvious advantage.3. The smell of the relationship between the medicinal effect to the identifica-tion of drug targets have obvious influence, its mechanism complex, needs to be further study, reveals its principle.4. In animal experiments, traditional Chinese medicine and western medicine, has certain advantages than because of its mechanism, but not like the western medicine, so, should be clear on the whole and evaluate its function and mech-anism, easy to reveal its biological basis and nature.