| Objective: Results from epidemic studies and laboratory tests indicated that consumption of allium vegetables may considerably reduce the relative risk of gastric carcinoma. Our previous experiments already proved that the extracts of Allium fistulosum.L can induce apoptosis of gastrocancerous cell and inhibit cell growth, especially lipid portion of extracts did. Our aim to study is further explore the possible signal molecule mechanism in apoptotic process of gastrocancerous cell MGC80-3 induced by Allium fistulosum.L oil (AFLO).Methods: (1) MGC80-3 cells were treated with AFLO in different concentrations for 24 hour. The optical density values (OD) were measured by MTT test. Then the growth inhibitory rates were counted and IC50 was measured. (2) MGC80-3 cells were incubated in nutritious fluid with AFLO (100u g/ml) for one hour to twelve hours. The treated cells were stained with Hoechst33258. Apoptotic shape was investigated with fluorescence microscope. (3) MGC80-3 cells were incubated in nutritious fluid, with AFLO (100u g/ml) for one hour to twelve hours. Total apoptotic rates andearly apoptotic rates were detected by measuring cellular PI and Annixin V fluorescence values on flow cytometry. (4) MGC80-3 cells were incubated in nutritious fluid with AFLO (100 u g/ml) for five minutes to twelve hours. Fura-2/AM was used as an intracellular-free calcium indicator. Intracellular-free calcium content at different time was determined by flow cytometry. (5) MGC80-3 cells were incubated in nutritious fluid with AFLO (100ug/ml) for five minutes to twelve hours. Intracellular cAMP levels were determined by radioimmunoassay (RIA).Results: (1) Effect of AFLO in different concentrations on gastrocancerous cellular growth; MGC80-3 cells were treated with 12.5 u g/ml, 25 u g/ml, 50 u g/ml, 75 u g/ml and 100 U g/ml of AFLO for 24 hours. With the concentration of AFLO increased, the optical density of test groups declined, but cellular growth inhibitory rates went up correspondingly and reached to 5.71%, 36.33%, 44.34%, 54.52% and74.15% respectively. IC50 65ug/ml. Effect of inhibition on gastrocancerous cellular growth depended on concentration of AFLO. There were not any like these changes in control group. (2) Observation for apoptotic gastrocancerous cellular shape: Hoechst 33258 fluorescent stain showed that block or article-liked fluorescence light appeared in nuclei or cytoplasm of some cells treated by AFLO (100u g/ml) for one hour to twelve hours. These imaginations indicted condensation and fragmentation of chromosome. Apoptosistook place in cells treated by AFLO , but fluorescence light in cells of control groups was scattered and well-distributed. (3) Changes of the apoptotic rates at different times: MGC80-3 cells were incubated in nutritious fluid with AFLO (100u g/ml) for Ih, 2h, 3h, 6h and 12h. The total apoptotic rates were 53.48%, 50.16%, 77.84%, 83.71% and 41.34% respectively. The early apoptotic rates were 50.15%, 33.25%, 11.69%, 4.65% and 0.84% respectively. The peak time of early apoptotic rate was at the first hour. (4) Changes of intracellular-free calcium content: MGC80-3 cells were treated by AFLO (100 u g/ml) for 5', 15', 30', Ih, 2h, 3h, 6h and 12h. Measured intracellular-free calcium content of test groups were 71.00+6.08, 79.67+1.53, 77.67+1.53, 83.33+5.03, 76.00+6.06, 72.33+4.24, 75.67+2.52, 65.00+2.00 respectively. Those in control groups were 48.67+3.06, 51.00+2.00, 49.6711.53, 48.06+1.53, 49.0012.65, 51.67+3.57, 47.6713.79 and 48.3312.52 respectively. The peak content of Ca2+ was reached at the first hour, then contents of Ca2+ declined gradually, but were still higher than in the control groups. (5) Changes of cAMP concentration: The gastrocancerous cells treated with AFLO(100 u g/ml) for 5', 15', 30', Ih, 2h, 3h, 6h and 12h, measured intracellular cAMP concentrations were 5.7610.36, 18.51+2.06, 20.40+1.65, 16.0911.46, 12.1610.78,9.8810.53,9.4710.78, 9.8211.06 (pmol/mg protein) respectively. Those in control groups were 5.8910.38, 6.0810.76, 5.9310.67, 5.1210.41,5.17...
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